Brandtzaeg P
Ann N Y Acad Sci. 1975 Jun 30;254:35-54. doi: 10.1111/j.1749-6632.1975.tb29154.x.
Anionic-exchange fractions of IgG labeled with FITC, MRITC, RB200SC, or RBITC were tested on different substrates, and the resultant fluorescence was evaluated with the Ploem optical system. Conjugations with MRITC or RB200SC were found to afford the following advantages over FITC: immunofluorescence sensitivity was elevated six to seven times on a molar basis; high sensitivity could be combined with a wide specificity interval; there was negligible fading of emitted light; there was negligible tissue autofluorescence at the excitation wavelength (546 nm); because of the two latter points, repeated observations could be made on tissue sections stored for several years; and eosinophilic leukocytes that are prone to yield nonspecific staining could easily by identified by switching to ultraviolet-blue light excitation.
用异硫氰酸荧光素（FITC）、四甲基罗丹明异硫氰酸酯（MRITC）、罗丹明B200磺酰氯（RB200SC）或罗丹明异硫氰酸酯（RBITC）标记的IgG的阴离子交换级分在不同底物上进行测试，并用普洛姆光学系统评估产生的荧光。发现与MRITC或RB200SC的偶联相对于FITC具有以下优点：免疫荧光敏感性在摩尔基础上提高了六到七倍；高敏感性可与宽特异性区间相结合；发射光的褪色可忽略不计；在激发波长（546nm）处组织自发荧光可忽略不计；由于后两点，可对保存数年的组织切片进行重复观察；通过切换到紫外-蓝光激发，容易识别容易产生非特异性染色的嗜酸性白细胞。
