线虫细胞操作的遗传方法。

Genetic Methods for Cellular Manipulation in C. elegans.

机构信息

Department of Biology, Technion- Israel Institute of Technology, Haifa, Israel.

出版信息

Methods Mol Biol. 2022;2468:51-72. doi: 10.1007/978-1-0716-2181-3_4.

PMID:35320560

Abstract

Neuron manipulation in vivo by ablation, activation or inactivation, and regulation of gene expression is essential for dissecting nervous system function. Here we describe genetic means for neuron manipulation in the nematode C. elegans, and provide protocols for generating transgenic animals containing these genetic tools.

摘要

体内神经元的消融、激活或失活以及基因表达的调控对于解析神经系统功能至关重要。在这里，我们描述了线虫 C. elegans 中神经元操作的遗传方法，并提供了生成含有这些遗传工具的转基因动物的方案。