秀丽隐杆线虫细胞操作的遗传方法。

Genetic Methods for Cellular Manipulations in C. elegans.

作者信息

Katz Menachem

机构信息

Laboratory of Developmental Genetics, The Rockefeller University, 810 Weiss Research Building, 1230 York Avenue, New York, NY, 10065, USA.

出版信息

Methods Mol Biol. 2015;1327:23-38. doi: 10.1007/978-1-4939-2842-2_3.

DOI:10.1007/978-1-4939-2842-2_3

PMID:26423965

Abstract

Neuron manipulation in vivo by ablation, activation, or inactivation, and regulation of gene expression, is essential for dissecting nervous system function. Here we describe genetic means for neuron manipulation in the nematode C. elegans, and provide protocols for generating transgenic animals containing these genetic tools.

摘要

通过消融、激活或失活在体内对神经元进行操控，以及对基因表达进行调控，对于剖析神经系统功能至关重要。在此，我们描述了线虫秀丽隐杆线虫中神经元操控的遗传学方法，并提供了生成包含这些遗传工具的转基因动物的方案。