用于生物技术应用的烟草花叶病毒结合肽的分离。
Isolation of Tobacco Mosaic Virus-Binding Peptides for Biotechnology Applications.
机构信息
Department of Nanoengineering, University of California San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA.
Department of NanoEngineering, Department of Bioengineering, Department of Radiology, Center for Nano-ImmunoEngineering, Moores Cancer Center, Institute for Materials Discovery and Design, University of California San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA.
出版信息
Chembiochem. 2022 Jun 3;23(11):e202200040. doi: 10.1002/cbic.202200040. Epub 2022 Apr 5.
Tobacco mosaic virus (TMV) was the first virus to be discovered and it is now widely used as a tool for biological research and biotechnology applications. TMV particles can be decorated with functional molecules by genetic engineering or bioconjugation. However, this can destabilize the nanoparticles, and/or multiple rounds of modification may be necessary, reducing product yields and preventing the display of certain cargo molecules. To overcome these challenges, we used phage display technology and biopanning to isolate a TMV-binding peptide (TBP ) with strong binding properties (IC =0.73 μM, K =0.16 μM), allowing the display of model cargos via a single mixing step. The TMV-binding peptide is specific for TMV but does not recognize free coat proteins and can therefore be used to decorate intact TMV or detect intact TMV particles in crude plant sap.
烟草花叶病毒（TMV）是第一个被发现的病毒，现在被广泛用作生物研究和生物技术应用的工具。TMV 颗粒可以通过基因工程或生物缀合来修饰功能分子。然而，这可能会使纳米颗粒不稳定，和/或需要多次修饰，从而降低产物产量，并阻止某些货物分子的展示。为了克服这些挑战，我们使用噬菌体展示技术和生物淘选来分离具有强结合特性的烟草花叶病毒结合肽（TBP）（IC=0.73 μM，K=0.16 μM），允许通过单一混合步骤来展示模型货物。TMV 结合肽特异性识别 TMV，但不识别游离外壳蛋白，因此可用于修饰完整的 TMV 或检测粗植物汁液中的完整 TMV 颗粒。
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