更好、更快、更便宜：冷冻电镜的最新进展。
Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy.
机构信息
New York Structural Biology Center, New York, NY, USA; email:
Simons Electron Microscopy Center, New York, NY, USA.
出版信息
Annu Rev Biochem. 2022 Jun 21;91:1-32. doi: 10.1146/annurev-biochem-032620-110705. Epub 2022 Mar 23.
Abstract
Cryo-electron microscopy (cryo-EM) continues its remarkable growth as a method for visualizing biological objects, which has been driven by advances across the entire pipeline. Developments in both single-particle analysis and in situ tomography have enabled more structures to be imaged and determined to better resolutions, at faster speeds, and with more scientists having improved access. This review highlights recent advances at each stageof the cryo-EM pipeline and provides examples of how these techniques have been used to investigate real-world problems, including antibody development against the SARS-CoV-2 spike during the recent COVID-19 pandemic.
摘要
低温电子显微镜（cryo-EM）作为一种可视化生物对象的方法继续快速发展，这得益于整个领域的进步。在单颗粒分析和原位断层摄影技术方面的发展，使得更多的结构能够以更快的速度、更高的分辨率成像和确定，并且更多的科学家能够更好地获得这些技术。本文综述了 cryo-EM 技术各个阶段的最新进展，并举例说明了这些技术如何用于解决实际问题，包括在最近的 COVID-19 大流行期间针对 SARS-CoV-2 刺突蛋白开发抗体。
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