Muster-Nassal C, Kolodner R
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7618-22. doi: 10.1073/pnas.83.20.7618.
Heteroduplex DNA substrates containing a 4- or 7-base-pair insertion/deletion mismatch or each of the eight possible single-base-pair mismatches were constructed. Extracts of mitotic Saccharomyces cerevisiae cells catalyzed the correction of mismatched nucleotides in a reaction that required Mg2+ and had a partial requirement for ATP and the four dNTPs. The insertion/deletion mismatches and the A X C and G X T mismatches were repaired efficiently, while the six other single-base-pair mismatches were repaired poorly or at undetectable rates. Mismatch correction was accompanied by the specific incorporation of less than 20 nucleotides at or near the site of the repaired mismatch.
构建了含有4或7个碱基对插入/缺失错配或八种可能的单碱基对错配中每一种的异源双链DNA底物。有丝分裂的酿酒酵母细胞提取物在需要Mg2+且部分需要ATP和四种dNTP的反应中催化错配核苷酸的校正。插入/缺失错配以及A×C和G×T错配被高效修复，而其他六种单碱基对错配修复效果较差或修复率无法检测。错配校正伴随着在修复的错配位点或其附近特异性掺入少于20个核苷酸。
