Gross L, Dreyfuss Y
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7928-31. doi: 10.1073/pnas.83.20.7928.
We have reported previously that the incidence of tumors induced in Sprague-Dawley rats by total-body gamma-ray irradiation can be considerably reduced by restriction of food intake [Gross, L. & Dreyfuss, Y. (1984) Proc. Natl. Acad. Sci. USA 81, 7596-7598]. In experiments reported here we investigated the influence of reduced food intake on the development of radiation-induced leukemia in C3H(f) mice. The incidence of spontaneous leukemia in mice of this strain does not exceed 0.5%, but it can be considerably increased by total-body x-irradiation. In our study, two groups of C3H(f) mice were submitted to fractionated total-body gamma-irradiation (150 rads, five times at weekly intervals; 1 rad = 0.01 gray). The first group received a full ad lib diet (4.5-5.4 g of Purina Rodent Lab Chow pellets per day, each). In this group 31 out of 58 females (53.4%) and 24 out of 50 males (48%) developed leukemia at an average age of 8 months. In the second group, consisting of sisters and brothers of the first group, and submitted to the same gamma-irradiation but receiving a restricted diet (2 g of Purina Lab Chow pellets each, followed by 3 g on alternate days), only 2 out of 55 females (3.6%), and 1 out of 36 males (2.8%), developed leukemia at an average age of 9 and 12 months, respectively. Leukemia in both groups was predominantly of the lymphatic or lymphoblastic form, the leukemic cells infiltrating most organs, particularly the thymus, mesenteric and peripheral lymph nodes, spleen, liver, kidneys, and bone marrow; in most instances the peripheral blood was also leukemic.
我们之前曾报道，通过限制食物摄入量，可大幅降低全身γ射线照射诱导的斯普拉格-道利大鼠肿瘤的发生率[格罗斯，L. & 德赖弗斯，Y.（1984年）《美国国家科学院院刊》81卷，7596 - 7598页]。在本文所报道的实验中，我们研究了食物摄入量减少对C3H(f)小鼠辐射诱导白血病发展的影响。该品系小鼠的自发性白血病发生率不超过0.5%，但全身X射线照射可使其显著增加。在我们的研究中，两组C3H(f)小鼠接受了分次全身γ射线照射（150拉德，每周5次；1拉德 = 0.01戈瑞）。第一组小鼠自由进食（每只每天4.5 - 5.4克普瑞纳啮齿动物实验室饲料颗粒）。在这组中，58只雌性小鼠中有31只（53.4%）、50只雄性小鼠中有24只（48%）在平均8个月龄时患上白血病。第二组由第一组的兄弟姐妹组成，接受相同的γ射线照射，但食物摄入量受限（每只先喂2克普瑞纳实验室饲料颗粒，然后隔天喂3克），在平均9个月龄和12个月龄时，55只雌性小鼠中只有2只（3.6%）、36只雄性小鼠中只有1只（2.8%）患上白血病。两组白血病主要为淋巴性或淋巴母细胞性，白血病细胞浸润大多数器官，特别是胸腺、肠系膜和外周淋巴结、脾脏、肝脏、肾脏和骨髓；大多数情况下外周血也呈白血病状态。
