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鉴定与功能分析为L.的花发育提供了见解

Identification and Function Analysis Provide Insights Into Flower Development of L.

作者信息

Zhao Yujie, Wang Yuying, Zhao Xueqing, Yan Ming, Ren Yuan, Yuan Zhaohe

机构信息

Co-innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.

College of Forestry, Nanjing Forestry University, Nanjing, China.

出版信息

Front Plant Sci. 2022 Mar 7;13:833747. doi: 10.3389/fpls.2022.833747. eCollection 2022.

DOI:10.3389/fpls.2022.833747
PMID:35321445
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8937018/
Abstract

Based on the genome and small-RNA sequencing of pomegranate, miRNA167 and three target genes were identified in "Taishanhong" genome. Three genes and their corresponding protein sequences, expression patterns in pomegranate flower development and under exogenous hormones treatments were systematically analyzed in this paper. We found that PgARF6s are nuclear proteins with conserved structures. However, PgARF6s had different protein structures and expression profiles in pomegranate flower development. At the critical stages of pomegranate ovule sterility (8.1-14.0 mm), the expression levels of in bisexual flowers were lower than those in functional male flowers. Interestingly, expression level was significantly higher than and . Under the treatment of exogenous IBA and 6-BA, were down-regulated, and the expression of was significantly inhibited. PgmiR167a and PgmiR167d had the binding site on genes sequences, and PgARF6a has the directly targeted regulatory relationship with PgmiR167a in pomegranate. At the critical stage of ovule development (8.1-12.0 mm), exogenous IBA and 6-BA promoted the content of GA and ZR accumulation, inhibited BR accumulation. There was a strong correlation between the expression of and . Under exogenous hormone treatment, the content of ZR, BR, GA, and ABA were negatively correlated with the expressions of genes. However, JA was positively correlated with and under IBA treatment. Thus, our results provide new evidence for genes involving in ovule sterility in pomegranate flowers.

摘要

基于石榴的基因组和小RNA测序，在“泰山红”基因组中鉴定出了miRNA167和三个靶基因。本文系统分析了这三个基因及其相应的蛋白质序列、在石榴花发育过程中和外源激素处理下的表达模式。我们发现PgARF6s是具有保守结构的核蛋白。然而，PgARF6s在石榴花发育过程中具有不同的蛋白质结构和表达谱。在石榴胚珠不育的关键阶段（8.1 - 14.0毫米），两性花中的表达水平低于功能性雄花。有趣的是，的表达水平显著高于和。在外源IBA和6 - BA处理下，被下调，的表达受到显著抑制。PgmiR167a和PgmiR167d在基因序列上具有结合位点，并且在石榴中PgARF6a与PgmiR167a具有直接靶向调控关系。在胚珠发育的关键阶段（8.1 - 12.0毫米），外源IBA和6 - BA促进了GA和ZR的积累，抑制了BR的积累。和的表达之间存在很强的相关性。在外源激素处理下，ZR、BR、GA和ABA的含量与基因的表达呈负相关。然而，在IBA处理下，JA与和呈正相关。因此，我们的结果为石榴花中涉及胚珠不育的基因提供了新的证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c0d/8937018/5097e9fedd16/fpls-13-833747-g013.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c0d/8937018/ef5bca75bf1a/fpls-13-833747-g011.jpg
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J Integr Plant Biol. 2022 Mar;64(3):702-716. doi: 10.1111/jipb.13197. Epub 2022 Feb 28.
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Phytohormones in fruit development and maturation.植物激素在果实发育和成熟中的作用。
Plant J. 2021 Jan;105(2):446-458. doi: 10.1111/tpj.15112. Epub 2021 Jan 12.
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Identification of Candidate Auxin Response Factors Involved in Pomegranate Seed Coat Development.
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Genome-wide identification and expression of YABBY genes family during flower development in Punica granatum L.石榴花发育过程中 YABBY 基因家族的全基因组鉴定和表达
Gene. 2020 Aug 20;752:144784. doi: 10.1016/j.gene.2020.144784. Epub 2020 May 18.
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Genome-wide identification of the ARF (auxin response factor) gene family in peach and their expression analysis.桃基因组中 ARF（生长素反应因子）基因家族的全基因组鉴定及其表达分析。
Mol Biol Rep. 2020 Jun;47(6):4331-4344. doi: 10.1007/s11033-020-05525-0. Epub 2020 May 19.
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