健康犬尿液脂质代谢产物特征。
The profile of urinary lipid metabolites in healthy dogs.
机构信息
Department of Animal Radiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
出版信息
J Vet Med Sci. 2022 May 1;84(5):644-647. doi: 10.1292/jvms.22-0020. Epub 2022 Mar 23.
Polyunsaturated fatty acids, including arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are converted to hundreds of lipid mediators by cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 (CYP), or through non-enzymatic processes, and they reflect inflammatory states of the body. We comprehensively analyzed lipid metabolites in dog urine using a liquid chromatograph-mass spectrometry (LC-MS/MS) to describe their metabolic characteristics. We detected 31 AA-derived metabolites, four EPA-derived metabolites, and a DHA-derived metabolite in all urine samples. Among AA-derived metabolites, 15, 5, 3, and 8 were generated by COX, LOX, CYP, and non-enzymatic oxidation respectively. This study will be the first step to use profiles of urinary lipid metabolites for better understanding and diagnosis of canine diseases.
多不饱和脂肪酸，包括花生四烯酸（AA）、二十二碳六烯酸（DHA）和二十碳五烯酸（EPA），可通过环氧化酶（COX）、脂氧合酶（LOX）和细胞色素 P450（CYP）转化为数百种脂质介质，或通过非酶促过程，它们反映了身体的炎症状态。我们使用液相色谱-质谱（LC-MS/MS）全面分析了狗尿中的脂质代谢物，以描述其代谢特征。我们在所有尿液样本中检测到 31 种 AA 衍生代谢物、4 种 EPA 衍生代谢物和 1 种 DHA 衍生代谢物。在 AA 衍生代谢物中，15、5、3 和 8 分别由 COX、LOX、CYP 和非酶氧化生成。这项研究将是首次使用尿脂质代谢物谱来更好地理解和诊断犬类疾病。
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