Center for Primary Care and Public Health (Unisanté), University of Lausanne, Route de la Corniche 2, 1066 Epalinges-Lausanne, Switzerland.
Center for Primary Care and Public Health (Unisanté), University of Lausanne, Route de la Corniche 2, 1066 Epalinges-Lausanne, Switzerland; Institut national de recherche et de sécurité (INRS), Vandoeuvre-lès-Nancy, France.
Toxicol Lett. 2020 Aug 1;328:19-27. doi: 10.1016/j.toxlet.2020.04.006. Epub 2020 Apr 19.
Oxidative stress is defined as an imbalance between the production and elimination of reactive oxygen species (ROS) are associated with various inflammation-related human disease. ROS can oxidize lipids, which subsequently undergo fragmentation to produce F2-isoprostanes (F2-IsoPs). Eight-isoprostane is one of the most extensively studied F2-IsoPs and the most commonly used biomarker for the assessment of oxidative stress in human studies. This urinary biomarker is quantified using either chemical or immunological techniques. A "physiological" range for 8-isoprostanes is needed to use this biomarker as a measure of excess oxidative stress originating from occupational exposures. However, ranges reported in the literature are inconsistent. We designed a standardized protocol of a systematic review and meta-analysis to assess baseline values for 8-isoprostane concentrations in urine of healthy adults and identify determinants of their inter- and intra-individual variability. We searched PubMed from journal inception and up to April 2019, and screened articles for studies containing F2-IsoPs concentrations in urine for healthy adult participants. We grouped studies in three biomarker groups: "8-isoprostane", "Isoprostanes" "15- F2t-Isoprostane". We computed geometric mean (GM) and geometric standard deviation (GSD) as the basis for the meta-analysis. Of the initial 1849 articles retrieved, 63 studies were included and 107 subgroups within these study populations were identified. We stratified the subgroups analyzed with the chemical methods by body mass index (BMI) reported. We provide pooled GM values for urinary 8-isoprostane concentrations in healthy adults, separately for chemical and immunological analysis in this review. The interquartile range (IQR) in subgroups with a mean BMI below 25 measured using chemical methods was 0.18 to 0.40 μg/g creatinine. We show that there is a significant positive association between BMI and urinary 8-isoprostane concentrations. We recommend adjusting urinary 8-isoprostane concentrations in spot urine with creatinine, quantifying 8-isoprostane with chemical analytical methods, and reporting results as median and quartiles. This will help in comparing results across studies.
氧化应激是指活性氧（ROS）的产生和消除之间的失衡，与各种与炎症相关的人类疾病有关。ROS 可以氧化脂质，随后脂质发生片段化产生 F2-异前列腺素（F2-IsoPs）。八异前列腺素是研究最广泛的 F2-IsoP 之一，也是评估人类氧化应激的最常用生物标志物。这种尿生物标志物使用化学或免疫学技术进行定量。需要“生理”范围内的 8-异前列腺素作为职业暴露引起的过量氧化应激的衡量标准。然而，文献中报道的范围不一致。我们设计了一个标准化的系统评价和荟萃分析方案，以评估健康成年人尿液中 8-异前列腺素浓度的基线值，并确定其个体内和个体间变异性的决定因素。我们从期刊创刊开始在 PubMed 中进行了搜索，截至 2019 年 4 月，并筛选了包含健康成年参与者尿液中 F2-IsoPs 浓度的文章。我们将研究分为三组生物标志物：“8-异前列腺素”、“异前列腺素”、“15-F2t-异前列腺素”。我们计算了几何平均值（GM）和几何标准差（GSD）作为荟萃分析的基础。在最初检索到的 1849 篇文章中，有 63 篇被纳入研究，这些研究人群中有 107 个亚组。我们根据报告的体重指数（BMI）对使用化学方法分析的亚组进行分层。我们在本综述中分别为化学和免疫学分析提供了健康成年人尿液中 8-异前列腺素浓度的汇总 GM 值。使用化学方法测量的平均 BMI 低于 25 的亚组的四分位间距（IQR）为 0.18 至 0.40 μg/g 肌酐。我们表明 BMI 与尿液中 8-异前列腺素浓度之间存在显著正相关。我们建议用肌酐调整尿液中 8-异前列腺素浓度，用化学分析方法定量 8-异前列腺素，并报告中位数和四分位数。这将有助于比较研究结果。
