State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, China.
College of Life Sciences, Yantai University, Yantai, China.
J Biomed Mater Res B Appl Biomater. 2022 Sep;110(9):2050-2062. doi: 10.1002/jbm.b.35059. Epub 2022 Mar 24.
Guided tissue regeneration (GTR) membranes play a vital role in periodontal surgery. Recently a series of composite electrospun membranes have been fabricated to improve the unexpected biodegradation of collagen-based GTR membranes. However, their tissue integrity needs to be studied in depth. In this study, a bi-layered electrospun membrane (BEM) inspired by "prodrug" was fabricated, which contained a dense-layer (BEM-DL) and a potential loose-layer (BEM-LL). The nanofibers of BEM-DL were composed of poly(l-lactic-co-glycolic acid) and tilapia skin collagen (TSC). Whereas the BEM-LL consisted of two types of nanofibers, one was the same as BEM-DL and the other was made from TSC. The morphology, degradation in vitro, cytocompatibility and biocompatibility in rats were investigated with a poly(lactic-co-glycolic acid) electrospun membrane (PLGA) as the negative control. The pore size of BEM-LL soaked for 7 days became larger than the original sample (164.8 ± 90.9 and 52.5 ± 21.0 μm , respectively), which was significantly higher (p < .05) than that of BEM-DL and PLGA. The BEM-LL displayed a larger weight loss rate of 82.3 ± 3.6% than the BEM-DL of 46.0 ± 2.8% at day 7 because of the rapid degradation of TSC fibers. The cytocompatibility test demonstrated that L929 cells were only spread on the surface of the BEM-DL while MC3T3-E1 cells grew into the BEM-LL layer. The subcutaneous implantation test further proved that BEM-DL performed as a cellular barrier, whereas BEM-LL was conducive to cell infiltration as deep as 200 μm with reduced fibrous encapsulation. Herein, the BEM inspired by "prodrug" is a promising GTR membrane with a property of enhanced tissue integration.
引导组织再生（GTR）膜在牙周手术中起着至关重要的作用。最近，一系列复合电纺膜已经被制造出来，以改善基于胶原蛋白的 GTR 膜的意外生物降解。然而，它们的组织完整性需要深入研究。在这项研究中，受“前药”启发，制备了一种双层电纺膜（BEM），包含致密层（BEM-DL）和潜在的疏松层（BEM-LL）。BEM-DL 的纳米纤维由聚（L-丙交酯-共-乙交酯）和罗非鱼皮胶原蛋白（TSC）组成。而 BEM-LL 由两种类型的纳米纤维组成，一种与 BEM-DL 相同，另一种由 TSC 制成。通过聚（L-丙交酯-共-乙交酯）电纺膜（PLGA）作为阴性对照，研究了 BEM 的形态、体外降解、细胞相容性和大鼠体内的生物相容性。BEM-LL 浸泡 7 天后的孔径比原始样品（分别为 164.8±90.9μm和 52.5±21.0μm）更大，差异具有统计学意义（p<0.05）。BEM-LL 在第 7 天的失重率为 82.3±3.6%，明显高于 BEM-DL 的 46.0±2.8%，这是由于 TSC 纤维的快速降解所致。细胞相容性试验表明，L929 细胞仅在 BEM-DL 表面展开，而 MC3T3-E1 细胞则进入 BEM-LL 层生长。皮下植入试验进一步证明，BEM-DL 起到细胞屏障的作用，而 BEM-LL 有利于细胞浸润至 200μm 深处，同时减少纤维包裹。因此，受“前药”启发的 BEM 是一种很有前途的 GTR 膜，具有增强组织整合的特性。
