Zhong Meiling, Lin Jixia, Yang Yudie, Liu Mingzhuo, Guo Guanghua, Ji Dehui, Zhang Richao, Zhang Jiali
58280East China Jiao Tong University, Nanchang, China.
117970First Affiliated Hospital of Nanchang University, Nanchang, China.
J Biomater Appl. 2022 Apr;36(9):1588-1598. doi: 10.1177/08853282211068596. Epub 2022 Feb 15.
Guided tissue regeneration (GTR) membranes have great potential to promote periodontal tissue regeneration and reestablishment. However, the regeneration potential and microbial infection resistance of current GTR membranes still need to be improved. Here, a bi-layered nanofibrous membrane on the basis of poly (lactic-co-glycolic acid) (PLGA)/gelatin with osteogenic and antibacterial functions was fabricated for periodontal tissue regeneration. The antimicrobial layer (AL) of the bi-layered nanofibrous membrane was composed of nanofibrous PLGA/gelatin nanofibers loaded with nano-silver (nAg), while the osteoconductive layer (OL) of the nanofibrous membrane consisted of PLGA/gelatin nanofibers loaded with nano-hydroxyapatite (nHA). The bi-layered nanofibrous membrane was examined by scanning electron microscopy (SEM), energy dispersive spectrometer (EDS), transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectrometry (XPS) and X-ray diffractometry (XRD). The results showed that nHA and nAg particles were well evenly loaded or embedded in PLGA/gelatin nanofibers. The cell culture experiments suggested that the bi-layered nanofibrous membrane possessed good cytocompatibility and the OL of the bi-layered nanofibrous membrane possessed an enhanced osteogenic capacity for human osteoblast-like cells (MG63), which was verified by the good cell viability and the increased alkaline phosphatase (ALP) activity, respectively. The results of antimicrobial study displayed that the AL of the bi-layered nanofibrous membrane possessed an effective antibacterial capability. In conclusion, the prepared bi-layered nanofibrous membrane with osteogenic and antibacterial functions may have great potential for periodontal tissue regeneration and reestablishment.[Formula: see text].
引导组织再生（GTR）膜在促进牙周组织再生和重建方面具有巨大潜力。然而，目前GTR膜的再生潜力和抗微生物感染能力仍有待提高。在此，制备了一种基于聚乳酸-乙醇酸共聚物（PLGA）/明胶的具有成骨和抗菌功能的双层纳米纤维膜用于牙周组织再生。双层纳米纤维膜的抗菌层（AL）由负载纳米银（nAg）的PLGA/明胶纳米纤维组成，而纳米纤维膜的骨传导层（OL）由负载纳米羟基磷灰石（nHA）的PLGA/明胶纳米纤维组成。通过扫描电子显微镜（SEM）、能量色散光谱仪（EDS）、透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）、X射线光电子能谱（XPS）和X射线衍射仪（XRD）对双层纳米纤维膜进行了检测。结果表明，nHA和nAg颗粒均匀地负载或嵌入在PLGA/明胶纳米纤维中。细胞培养实验表明，双层纳米纤维膜具有良好的细胞相容性，双层纳米纤维膜的OL对人成骨样细胞（MG63）具有增强的成骨能力，分别通过良好的细胞活力和增加的碱性磷酸酶（ALP）活性得到验证。抗菌研究结果表明，双层纳米纤维膜的AL具有有效的抗菌能力。总之，所制备的具有成骨和抗菌功能的双层纳米纤维膜在牙周组织再生和重建方面可能具有巨大潜力。[公式：见原文]
