Rogala Benjamin, Khan Zia Ali, Jackson-Boeters Linda, Darling Mark Roger
Department of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON N6A 5C1, Canada.
Dent J (Basel). 2022 Mar 4;10(3):38. doi: 10.3390/dj10030038.
Granular cell tumours (GCTs) are rare submucosal lesions, thought to develop from Schwann cells, characterised by large polygonal cells with abundant lysosomes. The objectives of this study are to investigate whether GCTs have an antigen-presenting cell (APC) phenotype or a neural crest phenotype using immunohistochemistry and to compare expression profiles with Schwannomas. Immunoreactivity to CD68, HLA-DR, CD163, CD40 and CD11c (APC phenotype) and markers of neural crest cell (NCC) origin S100, SOX10, NSE and GAP43 in 23 cases of GCTs and 10 cases of Schwannomas were evaluated. RT-qPCR was used to identify a possible NCC developmental phenotype in 6 cases of GCTs. GAP43 was identified as a new NCC marker for GCTs, and some evidence was found for an APC phenotype from CD68 and HLA-DR immunoreactivity. RT-qPCR failed to identify an NCC developmental phenotype of GCTs, likely due to technical issues.
颗粒细胞瘤（GCTs）是罕见的黏膜下病变，被认为起源于施万细胞，其特征是具有大量溶酶体的大的多边形细胞。本研究的目的是使用免疫组织化学研究颗粒细胞瘤是否具有抗原呈递细胞（APC）表型或神经嵴表型，并将其表达谱与神经鞘瘤进行比较。评估了23例颗粒细胞瘤和10例神经鞘瘤对CD68、HLA-DR、CD163、CD40和CD11c（APC表型）以及神经嵴细胞（NCC）起源标志物S100、SOX10、NSE和GAP43的免疫反应性。采用逆转录-定量聚合酶链反应（RT-qPCR）鉴定6例颗粒细胞瘤中可能的NCC发育表型。GAP43被确定为颗粒细胞瘤的一种新的NCC标志物，并且从CD68和HLA-DR免疫反应性中发现了一些支持APC表型的证据。RT-qPCR未能鉴定出颗粒细胞瘤的NCC发育表型，可能是由于技术问题。
