Khaing Ei Mon, Mahadlek Jongjan, Okonogi Siriporn, Phaechamud Thawatchai
Programme of Pharmaceutical Engineering, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand.
Pharmaceutical Intelligence Unit Prachote Plengwittaya, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand.
Gels. 2022 Mar 8;8(3):169. doi: 10.3390/gels8030169.
Localized intra-periodontal pocket drug delivery using an injectable in situ forming gel is an effective periodontitis treatment. The aqueous insoluble property of rosin is suitable for preparing a solvent exchange-induced in situ forming gel. This study aims to investigate the role of incorporating lime peel oil (LO) on the physicochemical properties of injectable in situ forming gels based on rosin loaded with 5% / doxycycline hyclate (DH) in dimethyl sulfoxide (DMSO) and -methyl pyrrolidone (NMP). Their gel formation, viscosity, injectability, mechanical properties, wettability, drug release, and antimicrobial activities were evaluated. The presence of LO slowed gel formation due to the loose precipitate formation of gel with a high LO content. The viscosity and injectability were slightly increased with higher LO content for the DH-loaded rosin-based in situ forming gel. The addition of 10% LO lowered gel hardness with higher adhesion. LO incorporation promoted a higher drug release pattern than the no oil-added formulation over 10 days and the gel formation rate related to burst drug release. The drug release kinetics followed the non-Fickian diffusion mechanism for oil-added formulations. LO exhibited high antimicrobial activity against and . The DH-loaded rosin in situ forming gel with an addition of LO (0, 2.5, 5, and 10% /) inhibited all tested microorganisms. Adding 10% LO to rosin-based in situ forming gel improved the antimicrobial activities, especially for the and As a result, the study demonstrates the possibility of using an LO amount of less than 10% loading into a rosin-based in situ forming gel for efficient periodontitis treatment.
使用可注射原位形成凝胶进行局部牙周袋内药物递送是一种有效的牙周炎治疗方法。松香的水不溶性特性适合制备溶剂交换诱导的原位形成凝胶。本研究旨在探讨添加酸橙皮油（LO）对基于负载5%盐酸多西环素（DH）的松香在二甲基亚砜（DMSO）和N-甲基吡咯烷酮（NMP）中的可注射原位形成凝胶的物理化学性质的作用。评估了它们的凝胶形成、粘度、可注射性、机械性能、润湿性、药物释放和抗菌活性。由于高LO含量的凝胶形成松散沉淀，LO的存在减缓了凝胶形成。对于负载DH的松香基原位形成凝胶，随着LO含量的增加，粘度和可注射性略有增加。添加10%的LO降低了凝胶硬度，但具有更高的粘附性。在10天内，与无油添加配方相比，添加LO促进了更高的药物释放模式，并且凝胶形成速率与药物突释有关。添加油的配方的药物释放动力学遵循非菲克扩散机制。LO对[具体菌种1]和[具体菌种2]表现出高抗菌活性。添加LO（0、2.5、5和10%/）的负载DH的松香原位形成凝胶抑制了所有测试的微生物。向松香基原位形成凝胶中添加10%的LO提高了抗菌活性，尤其是对[具体菌种1]和[具体菌种2]。因此，该研究证明了在松香基原位形成凝胶中使用低于10%的LO量进行有效牙周炎治疗的可能性。
