Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA.
Departments of Medicine, Division of Medical Genetics and Genome Sciences, University of Washington, Seattle, WA 98195, USA.
Biosensors (Basel). 2022 Feb 28;12(3):149. doi: 10.3390/bios12030149.
Current point-of-care (POC) screening of Coronavirus disease 2019 (COVID-19) requires further improvements to achieve highly sensitive, rapid, and inexpensive detection. Here we describe an immunoresistive sensor on a polyethylene terephthalate (PET) film for simple, inexpensive, and highly sensitive COVID-19 screening. The sensor is composed of single-walled carbon nanotubes (SWCNTs) functionalized with monoclonal antibodies that bind to the spike protein of SARS-CoV-2. Silver electrodes are silkscreen-printed on SWCNTs to reduce contact resistance. We determine the SARS-CoV-2 status via the resistance ratio of control- and SARS-CoV-2 sensor electrodes. A combined measurement of two adjacent sensors enhances the sensitivity and specificity of the detection protocol. The lower limit of detection (LLD) of the SWCNT assay is 350 genome equivalents/mL. The developed SWCNT sensor shows 100% sensitivity and 90% specificity in clinical sample testing. Further, our device adds benefits of a small form factor, simple operation, low power requirement, and low assay cost. This highly sensitive film sensor will facilitate rapid COVID-19 screening and expedite the development of POC screening platforms.
目前，针对 2019 年冠状病毒病（COVID-19）的即时护理（POC）筛查需要进一步改进，以实现高灵敏度、快速和廉价的检测。在这里，我们描述了一种在聚对苯二甲酸乙二醇酯（PET）薄膜上的免疫电阻传感器，用于简单、廉价和高灵敏度的 COVID-19 筛查。该传感器由单壁碳纳米管（SWCNTs）组成，其表面功能化有与 SARS-CoV-2 的刺突蛋白结合的单克隆抗体。银电极通过丝印在 SWCNTs 上来降低接触电阻。我们通过对照和 SARS-CoV-2 传感器电极的电阻比来确定 SARS-CoV-2 的状态。两个相邻传感器的组合测量提高了检测方案的灵敏度和特异性。SWCNT 分析的检测限（LOD）低至 350 个基因组当量/mL。该开发的 SWCNT 传感器在临床样本测试中显示出 100%的灵敏度和 90%的特异性。此外，我们的设备还具有体积小、操作简单、功耗低和检测成本低等优点。这种高灵敏度的薄膜传感器将促进 COVID-19 的快速筛查，并加速 POC 筛查平台的发展。
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