Henderson Ben, Meurs Joris, Lamers Carlijn R, Batista Guilherme Lopes, Materić Dušan, Bertinetto Carlo G, Bongers Coen C W G, Holzinger Rupert, Harren Frans J M, Jansen Jeroen J, Hopman Maria T E, Cristescu Simona M
Department of Analytical Chemistry and Chemometrics, Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, The Netherlands.
Division of Human Nutrition and Health, Wageningen University & Research, 6708 WE Wageningen, The Netherlands.
Metabolites. 2022 Mar 3;12(3):224. doi: 10.3390/metabo12030224.
The aim of this study was to investigate volatile organic compounds (VOCs) in exhaled breath as possible non-invasive markers to monitor the inflammatory response in inflammatory bowel disease (IBD) patients as a result of repeated and prolonged moderate-intensity exercise. We included 18 IBD patients and 19 non-IBD individuals who each completed a 30, 40, or 50 km walking exercise over three consecutive days. Breath and blood samples were taken before the start of the exercise event and every day post-exercise to assess changes in the VOC profiles and cytokine concentrations. Proton transfer reaction time-of-flight mass spectrometry (PTR-ToF-MS) was used to measure exhaled breath VOCs. Multivariate analysis, particularly ANOVA-simultaneous component analysis (ASCA), was employed to extract relevant ions related to exercise and IBD. Prolonged exercise induces a similar response in breath butanoic acid and plasma cytokines for participants with or without IBD. Butanoic acid showed a significant correlation with the cytokine IL-6, indicating that butanoic acid could be a potential non-invasive marker for exercise-induced inflammation. The findings are relevant in monitoring personalized IBD management.
本研究的目的是调查呼出气中的挥发性有机化合物（VOCs），作为可能的非侵入性标志物，以监测炎症性肠病（IBD）患者因反复进行长时间中等强度运动而产生的炎症反应。我们纳入了18名IBD患者和19名非IBD个体，他们每人在连续三天内完成了30、40或50公里的步行运动。在运动开始前以及运动后的每一天采集呼吸和血液样本，以评估VOC谱和细胞因子浓度的变化。采用质子转移反应飞行时间质谱（PTR-ToF-MS）测量呼出气中的VOCs。采用多变量分析，特别是方差分析-同步成分分析（ASCA），来提取与运动和IBD相关的相关离子。对于有或没有IBD的参与者，长时间运动在呼出丁酸和血浆细胞因子方面诱导了类似的反应。丁酸与细胞因子IL-6显示出显著相关性，表明丁酸可能是运动诱导炎症的潜在非侵入性标志物。这些发现对于监测个性化的IBD管理具有重要意义。
