Tokuoka Suzumi M, Kita Yoshihiro, Sato Masaya, Shimizu Takao, Yatomi Yutaka, Oda Yoshiya
Department of Lipidomics, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8654, Japan.
Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8654, Japan.
Metabolites. 2022 Mar 5;12(3):230. doi: 10.3390/metabo12030230.
Blood samples are minimally invasive and can be collected repeatedly, but they are far from the site of disease and the target molecules are diluted by the large amount of blood. Therefore, we performed lipidomics using immunoprecipitation as a method to enrich specific fractions of serum. In this study, a CD9 antibody was immobilized on magnetic beads to enrich CD9-containing components in the serum for lipidomics. The percentages of phospholipids recovered from serum by methanol and isopropanol extractions were not significantly different, but triglycerides were barely recovered from serum by methanol extraction, requiring the use of isopropanol. However, once the serum was enriched with CD9 magnetic beads, triglycerides, and phospholipids were recovered at similar levels in both methanol and isopropanol extractions. Therefore, it is possible that the triglyceride fraction of the whole serum and the triglyceride fraction were enriched in CD9 magnetic beads differ in localization and properties. In addition, the variation per disease was small in general serum lipidomics; however, the difference per disease appeared larger when CD9 magnetic bead enrichment was employed.
血液样本具有微创性且可重复采集，但它们远离疾病部位，目标分子会被大量血液稀释。因此，我们采用免疫沉淀法进行脂质组学研究，以富集血清中的特定组分。在本研究中，将CD9抗体固定在磁珠上，用于富集血清中含CD9的组分以进行脂质组学分析。甲醇和异丙醇萃取从血清中回收的磷脂百分比无显著差异，但甲醇萃取几乎无法从血清中回收甘油三酯，因此需要使用异丙醇。然而，一旦血清用CD9磁珠富集，甲醇和异丙醇萃取中甘油三酯和磷脂的回收水平相似。因此，全血清中的甘油三酯组分与在CD9磁珠中富集的甘油三酯组分在定位和性质上可能存在差异。此外，在一般血清脂质组学中，每种疾病的变化通常较小；然而，采用CD9磁珠富集时，每种疾病的差异似乎更大。
