Cant Amélie, Bonnard Marc, Porcher Jean-Marc, Prygiel Jean, Catteau Audrey, Delahaut Laurence, Palluel Olivier, Turiès Cyril, Geffard Alain, Bado-Nilles Anne
Institut National de l'Environnement Industriel et des Risques (INERIS), UMR-I 02 SEBIO, Parc Technologique Alata, BP 2, 60550 Verneuil-en-Halatte, France.
Université de Reims Champagne-Ardenne (URCA), UMR-I 02 SEBIO, UFR Sciences Exactes et Naturelles, Campus Moulin de la Housse, BP 1039, 51687 Reims, France.
Toxics. 2022 Feb 22;10(3):101. doi: 10.3390/toxics10030101.
Water is impacted by a variety of increasing pressures, such as contaminants, including genotoxic pollutants. The proposed multi-biomarker approach at a sub-individual level gives a complementary indicator to the chemical and ecological parameters of the Water Framework Directive (WFD, 2000/60/EC). By integrating biomarkers of genotoxicity and erythrocyte necrosis in the sentinel fish species the three-spined stickleback () through active biomonitoring of six stations of the Artois-Picardie watershed, north France, our work aimed to improve the already existing biomarker approach. Even if fish in all stations had high levels of DNA strand breaks, the multivariate analysis (PCA), followed by hierarchical agglomerative clustering (HAC), improved discrimination among stations by detecting an increase of nuclear DNA content variation (, , and ) and erythrocyte necrosis (, ). The present work highlighted that the integration of these biomarkers of genotoxicity in a multi-biomarker approach is appropriate to expand physiological parameters which allow the targeting of new potential effects of contaminants.
水受到各种不断增加的压力影响，例如污染物，包括具有基因毒性的污染物。所提议的个体水平以下的多生物标志物方法为《水框架指令》（WFD，2000/60/EC）的化学和生态参数提供了一个补充指标。通过在法国北部阿图瓦 - 皮卡第流域的六个站点对哨兵鱼类物种三刺鱼（）进行基因毒性和红细胞坏死生物标志物的整合，通过主动生物监测，我们的工作旨在改进现有的生物标志物方法。即使所有站点的鱼类都有高水平的DNA链断裂，但多变量分析（PCA），随后进行层次凝聚聚类（HAC），通过检测核DNA含量变化（、、和）以及红细胞坏死（、）的增加，改善了站点间的区分。目前的工作强调，在多生物标志物方法中整合这些基因毒性生物标志物适用于扩展生理参数，从而能够针对污染物的新潜在影响。
