Petrilla Janka, Mátis Gábor, Mackei Máté, Kulcsár Anna, Sebők Csilla, Papp Márton, Gálfi Péter, Fébel Hedvig, Huber Korinna, Neogrády Zsuzsanna
Division of Biochemistry, Department of Physiology and Biochemistry, University of Veterinary Medicine, 1078 Budapest, Hungary.
Center for Bioinformatics, University of Veterinary Medicine, 1078 Budapest, Hungary.
Vet Sci. 2022 Feb 25;9(3):103. doi: 10.3390/vetsci9030103.
Influencing the endocrine metabolic regulation of chickens by nutritional factors might provide novel possibilities for improving animal health and productivity. This study was designed to evaluate the impact of dietary cereal type (wheat-based (WB) vs. maize-based (MB) diets), crude protein level (normal (NP) vs. lowered (LP)), and sodium (n-)butyrate (1.5 g/kg diet) supplementation (vs. no butyrate) on the responsiveness of hepatic glucagon receptor (GCGR), insulin receptor beta (IRβ) and mammalian target of rapamycin (mTOR) in the phase of intensive growth of chickens. Liver samples of Ross 308 broiler chickens () were collected on day 21 for quantitative real-time polymerase chain reaction and Western blot analyses. Hepatic GCGR and mTOR gene expressions were up-regulated by WB and LP diet. GCGR and IRβ protein level decreased in groups with butyrate supplementation; however, the quantity of IRβ and mTOR protein increased in WB groups. Based on these data, the applied dietary strategies may be useful tools to modulate hepatic insulin and glucagon signaling of chickens in the period of intensive growth. The obtained results might contribute to the better understanding of glycemic control of birds and increase the opportunity of ameliorating insulin sensitivity, hence, improving the production parameters and the welfare of broilers.
通过营养因素影响鸡的内分泌代谢调节可能为改善动物健康和生产力提供新的可能性。本研究旨在评估日粮谷物类型（小麦型（WB）与玉米型（MB）日粮）、粗蛋白水平（正常（NP）与降低（LP））以及添加丁酸钠（1.5 g/kg日粮）（与不添加丁酸钠相比）对鸡快速生长阶段肝脏胰高血糖素受体（GCGR）、胰岛素受体β（IRβ）和雷帕霉素靶蛋白（mTOR）反应性的影响。在第21天采集罗斯308肉鸡的肝脏样本进行定量实时聚合酶链反应和蛋白质印迹分析。WB和LP日粮上调了肝脏GCGR和mTOR基因表达。添加丁酸钠的组中GCGR和IRβ蛋白水平降低；然而，WB组中IRβ和mTOR蛋白量增加。基于这些数据，所应用的日粮策略可能是调节鸡快速生长阶段肝脏胰岛素和胰高血糖素信号的有用工具。所得结果可能有助于更好地理解禽类的血糖控制，并增加改善胰岛素敏感性的机会，从而改善生产性能和肉鸡的福利。
