Martínez-Orellana Pamela, González Noemí, Baldassarre Antonella, Álvarez-Fernández Alejandra, Ordeix Laura, Paradies Paola, Soto Manuel, Solano-Gallego Laia
Departament de Medicina i Cirurgia Animal, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Dipartimento delle Emergenze e dei Trapianti d' Organo, Sezione Cliniche Veterinarie, Università degli Studi di Bari Aldo Moro, 70124 Bari, Italy.
Vet Sci. 2022 Mar 4;9(3):116. doi: 10.3390/vetsci9030116.
The effect of Leishmania infantum soluble antigen (LSA) and recombinant Kinetoplastid Membrane Protein 11 (rKMP11) on the induction of ex vivo specific IFN-γ (n = 69) and antibody responses (n = 108) was determined in dogs. All dogs were tested for serological response to both antigens and divided into Group 1: healthy (Asturias, Spain, n = 26), Group 2: sick (n = 46), Group 3: healthy Ibizan hounds (Mallorca, Spain, n = 22) and Group 4: healthy (Bari, Italy, n = 14). Antibody levels were higher for LSA when compared to rKMP11 (p = 0.001). Ibizan hounds were all seronegative to rKMP11 and 18% were low seropositive to LSA. Sick dogs presented higher antibody response to both antigens compared to the rest of the groups (p < 0.0001). All groups showed higher IFN-γ levels after LSA compared to rKMP11 responses (p < 0.05). The highest response to LSA was found in Ibizan hounds (p < 0.05). IFN-γ to LSA and rKMP11 stimulation was observed in 34% and in 2.8% of the sick dogs, respectively. Here, we demonstrated that anti-rKMP11 antibodies are mainly present in dogs with moderate to severe disease. Furthermore, cellular immune response measured by specific ex vivo IFN-γ production was more intense to LSA than stimulated to rKMP11.
在犬类中测定了婴儿利什曼原虫可溶性抗原（LSA）和重组动基体膜蛋白11（rKMP11）对体外特异性干扰素-γ（n = 69）诱导及抗体反应（n = 108）的影响。对所有犬类进行了针对这两种抗原的血清学反应检测，并将其分为1组：健康犬（西班牙阿斯图里亚斯，n = 26），2组：患病犬（n = 46），3组：健康伊维萨猎犬（西班牙马略卡岛，n = 22）和4组：健康犬（意大利巴里，n = 14）。与rKMP11相比，LSA的抗体水平更高（p = 0.001）。伊维萨猎犬对rKMP11均为血清阴性，对LSA有18%为低血清阳性。与其他组相比，患病犬对两种抗原均呈现出更高的抗体反应（p < 0.0001）。与rKMP11反应相比，所有组在LSA刺激后均显示出更高的干扰素-γ水平（p < 0.05）。在伊维萨猎犬中发现对LSA的反应最高（p < 0.05）。分别在34%和2.8%的患病犬中观察到对LSA和rKMP11刺激产生的干扰素-γ。在此，我们证明抗rKMP11抗体主要存在于中度至重度疾病的犬类中。此外，通过体外特异性干扰素-γ产生测定的细胞免疫反应对LSA的强度比对rKMP11刺激的更强。
