Graduate Institute of Biomedical Optomechatronics, Taipei Medical University, Taipei 110, Taiwan.
Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.
Cells. 2022 Mar 11;11(6):972. doi: 10.3390/cells11060972.
Photobiomodulation (PBM) has recently emerged in cellular therapy as a potent alternative in promoting cell proliferation, migration, and differentiation during tissue regeneration. Herein, a single-cell near-infrared (NIR) laser irradiation system (830 nm) and the image-based approaches were proposed for the investigation of the modulatory effects in mitochondrial membrane potential (ΔΨm), reactive oxygen species (ROS), and vesicle transport in single living human adipose mesenchymal stem cells (hADSCs). The irradiated-hADSCs were then stained with 2',7'-dichlorodihydrofluorescein diacetate (HDCFDA) and Rhodamine 123 (Rh123) to represent the ΔΨm and ROS production, respectively, with irradiation in the range of 2.5-10 (J/cm), where time series of bright-field images were obtained to determine the vesicle transport phenomena. Present results showed that a fluence of 5 J/cm of PBM significantly enhanced the ΔΨm, ROS, and vesicle transport phenomena compared to the control group (0 J/cm) after 30 min PBM treatment. These findings demonstrate the efficacy and use of PBM in regulating ΔΨm, ROS, and vesicle transport, which have potential in cell proliferation, migration, and differentiation in cell-based therapy.
光生物调节（PBM）最近在细胞治疗中崭露头角，成为促进组织再生过程中细胞增殖、迁移和分化的有效替代方法。本文提出了一种单细胞近红外（NIR）激光辐照系统（830nm）和基于图像的方法，用于研究单个活人体脂肪间充质干细胞（hADSCs）中线粒体膜电位（ΔΨm）、活性氧（ROS）和囊泡运输的调节作用。辐照后的 hADSCs 用 2'，7'-二氯二氢荧光素二乙酸酯（HDCFDA）和罗丹明 123（Rh123）染色，分别代表 ΔΨm 和 ROS 的产生，辐照范围为 2.5-10（J/cm），在此期间获得一系列亮场图像以确定囊泡运输现象。目前的结果表明，与对照组（0 J/cm）相比，PBM 的 5 J/cm 辐照显著增强了 30 分钟 PBM 处理后的 ΔΨm、ROS 和囊泡运输现象。这些发现证明了 PBM 在调节 ΔΨm、ROS 和囊泡运输方面的功效和用途，这在基于细胞的治疗中对细胞增殖、迁移和分化具有潜在作用。
