Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 110, Taiwan.
Graduate Institute of Biomedical Optomechatronics, Taipei Medical University, Taipei 110, Taiwan; Division of Plastic Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei 110, Taiwan; International PhD Program in Medicine, Taipei Medical University, Taipei 110, Taiwan.
J Photochem Photobiol B. 2024 Jul;256:112940. doi: 10.1016/j.jphotobiol.2024.112940. Epub 2024 May 14.
Photobiomodulation (PBM) is a well-established medical technology that employs diverse light sources like lasers or light-emitting diodes to generate diverse photochemical and photophysical reactions in cells, thereby producing beneficial clinical outcomes. In this study, we introduced an 830 nm near-infrared (NIR) laser irradiation system combined with a microscope objective to precisely and controllably investigate the impact of PBM on the migration and viability of human adipose mesenchymal stem cells (hADSCs). We observed a biphasic dose-response in hADSCs' viability and migration after PBM exposure (0-10 J/cm), with the 5 J/cm group showing significantly higher cell viability and migration ability than other groups. Additionally, at the optimal dose of 5 J/cm, we used nanoparticle tracking analysis (NTA) and found a 6.25-fold increase in the concentration of extracellular vesicles (EVs) derived from hADSCs (PBM/ADSC-EVs) compared to untreated cells (ADSC-EVs). Both PBM/ADSC-EVs and ADSC-EVs remained the same size, with an average diameter of 56 nm measured by the ExoView R200 system, which falls within the typical size range for exosomes. These findings demonstrate that PBM not only improves the viability and migration of hADSCs but also significantly increases the EV yield.
光生物调节（PBM）是一种成熟的医疗技术，它采用各种光源，如激光或发光二极管，在细胞中产生各种光化学和光物理反应，从而产生有益的临床效果。在这项研究中，我们引入了一个 830nm 近红外（NIR）激光辐照系统，结合显微镜物镜，精确地和可控地研究 PBM 对人脂肪间充质干细胞（hADSCs）迁移和活力的影响。我们观察到 PBM 暴露后 hADSCs 的活力和迁移呈双相剂量反应（0-10J/cm），5J/cm 组的细胞活力和迁移能力明显高于其他组。此外，在最佳剂量 5J/cm 下，我们使用纳米颗粒跟踪分析（NTA）发现，与未经处理的细胞（ADSC-EVs）相比，源自 hADSCs 的细胞外囊泡（EVs）的浓度增加了 6.25 倍（PBM/ADSC-EVs）。PBM/ADSC-EVs 和 ADSC-EVs 的大小相同，通过 ExoView R200 系统测量的平均直径为 56nm，这在典型的外泌体大小范围内。这些发现表明，PBM 不仅提高了 hADSCs 的活力和迁移能力，而且显著增加了 EV 的产量。
