Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Johannesburg, 2028, South Africa.
Lasers Med Sci. 2024 Oct 11;39(1):257. doi: 10.1007/s10103-024-04172-2.
Adipose-derived mesenchymal stem cells (ADMSCs) possess the ability to transform into various cell types, including neurons. It has been proposed that the optimization of this transformation can be achieved by using photobiomodulation (PBM). The objective of this laboratory-based investigation was to induce the transformation of immortalized ADMSCs (iADMSCs) into neurons with chemical triggers and then evaluate the supportive effects of PBM at two different wavelengths, 525 nm and 825 nm, each administered at a dose of 5 J/cm, as well as the combined application of these wavelengths. The results revealed that the treated cells retained their stem cell characteristics, although the cells exposed to the green laser exhibited a reduction in the CD44 marker. Furthermore, early, and late neuronal markers were identified using flow cytometry analysis. The biochemical analysis included the assessment of cell morphology, viability, cell proliferation, potential cytotoxicity, and the generation of reactive oxygen species (ROS). The findings of this study indicate that PBM does not harm the differentiation process and may even enhance it, but it necessitates a longer incubation period in the induction medium. These research findings contribute to the validation of stem cell technology for potential applications in in vivo, pre-clinical, and clinical research environments.
脂肪间充质干细胞（ADMSCs）具有转化为多种细胞类型的能力，包括神经元。有人提出，通过光生物调节（PBM）可以优化这种转化。本实验室研究的目的是用化学触发剂诱导永生化 ADMSCs（iADMSCs）向神经元转化，然后评估两种不同波长的 PBM 的支持作用，波长分别为 525nm 和 825nm，每个剂量为 5J/cm，并结合应用这两种波长。结果表明，处理后的细胞保留了其干细胞特征，尽管暴露于绿光的细胞中 CD44 标志物减少。此外，还使用流式细胞术分析鉴定了早期和晚期神经元标志物。生化分析包括评估细胞形态、活力、细胞增殖、潜在细胞毒性和活性氧（ROS）的产生。这项研究的结果表明，PBM 不会损害分化过程，甚至可能增强它，但需要在诱导培养基中延长孵育时间。这些研究结果为干细胞技术在体内、临床前和临床研究环境中的潜在应用提供了验证。
