Gambino Delia, Savoca Dario, Sucato Arianna, Gargano Valeria, Gentile Antonino, Pantano Licia, Vicari Domenico, Alduina Rosa
Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", 90129 Palermo, Italy.
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90028 Palermo, Italy.
Antibiotics (Basel). 2022 Mar 3;11(3):332. doi: 10.3390/antibiotics11030332.
Seawater could be considered a reservoir of antibiotic-resistant bacteria and antibiotic resistance genes. In this communication, we evaluated the presence of bacterial strains in seawater collected from different coasts of Sicily by combining microbiological and molecular methods. Specifically, we isolated viable bacteria that were tested for their antibiotic resistance profile and detected both antibiotic and heavy metal resistance genes. Both antibiotic-resistant Gram-negative bacteria, and , and specific antibiotic resistance genes were found in the seawater samples. Alarming levels of resistance were determined towards cefazolin, streptomycin, amoxicillin/clavulanic acid, ceftriaxone, and sulfamethoxazole/trimethoprim, and mainly genes conferring resistance to β-lactamic and sulfonamide antibiotics were detected. This survey, on the one hand, presents a picture of the actual situation, showing the pollution status of the Tyrrhenian coast of Sicily, and, on the other hand, can be considered as a baseline to be used as a reference time for future analysis.
海水可被视为抗药细菌和抗生素抗性基因的储存库。在本通讯中，我们通过结合微生物学和分子方法，评估了从西西里岛不同海岸采集的海水中细菌菌株的存在情况。具体而言，我们分离出了有活力的细菌，检测了它们的抗生素抗性谱，并检测了抗生素和重金属抗性基因。在海水样本中发现了耐抗生素的革兰氏阴性菌以及特定的抗生素抗性基因。对头孢唑林、链霉素、阿莫西林/克拉维酸、头孢曲松和磺胺甲恶唑/甲氧苄啶的抗性水平令人担忧，并且主要检测到了赋予对β-内酰胺类和磺胺类抗生素抗性的基因。这项调查一方面呈现了实际情况，展示了西西里岛第勒尼安海岸的污染状况，另一方面可被视为用作未来分析参考时间的基线。
