Sucato Arianna, Vecchioni Luca, Savoca Dario, Presentato Alessandro, Arculeo Marco, Alduina Rosa
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze, Bd. 16, 90128 Palermo, Italy.
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi, 18, 90123 Palermo, Italy.
Biology (Basel). 2021 Mar 6;10(3):200. doi: 10.3390/biology10030200.
In this study, we evaluated the microbiome and the resistome profile of water and fragments of polyethylene (PE) waste collected at the same time from a stream and the seawater in a coastal area of Northwestern Sicily. Although a core microbiome was determined by sequencing of the V3-V4 region of the bacterial 16S rDNA gene, quantitative differences were found among the microbial communities on PE waste and the corresponding water samples. Our findings indicated that PE waste contains a more abundant and increased core microbiome diversity than the corresponding water samples. Moreover, PCR analysis of specific antibiotic resistance genes (ARGs) showed that PE waste harbors more ARGs than the water samples. Thus, PE waste could act as a carrier of antibiotic-resistant microbiota, representing an increased danger for the marine environment and living organisms, as well.
在本研究中，我们评估了从西西里岛西北部沿海地区的一条溪流和海水中同时采集的水和聚乙烯（PE）废弃物碎片的微生物组和耐药基因组概况。尽管通过对细菌16S rDNA基因的V3-V4区域进行测序确定了一个核心微生物组，但在PE废弃物上的微生物群落与相应的水样之间发现了数量差异。我们的研究结果表明，PE废弃物所含的核心微生物组比相应的水样更丰富，多样性也更高。此外，对特定抗生素抗性基因（ARGs）的PCR分析表明，PE废弃物中携带的ARGs比水样更多。因此，PE废弃物可能充当抗生素抗性微生物群的载体，对海洋环境和生物也构成更大的危险。
