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来自[具体来源未提及]黏液的抗菌肽组分对NBIMCC 8785的作用及机制

Effect and Mechanisms of Antibacterial Peptide Fraction from Mucus of against NBIMCC 8785.

作者信息

Topalova Yana, Belouhova Mihaela, Velkova Lyudmila, Dolashki Aleksandar, Zheleva Nellie, Daskalova Elmira, Kaynarov Dimitar, Voelter Wolfgang, Dolashka Pavlina

机构信息

Faculty of Biology, Sofia University, 8 Dragan Tzankov Blvd., 1164 Sofia, Bulgaria.

Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 9, 1113 Sofia, Bulgaria.

出版信息

Biomedicines. 2022 Mar 14;10(3):672. doi: 10.3390/biomedicines10030672.

DOI:10.3390/biomedicines10030672
PMID:35327474
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8945727/
Abstract

Peptides isolated from the mucus of Cornu aspersum could be prototypes for antibiotics against pathogenic bacteria. Information regarding the mechanisms, effective concentration, and methods of application is an important tool for therapeutic, financial, and ecological regulation and a holistic approach to medical treatment. A peptide fraction with MW < 10 kDa was analyzed by MALDI-TOF-TOF using Autoflex™ III. The strain Escherichia coli NBIMCC 8785 (18 h and 48 h culture) was used. The changes in bacterial structure and metabolic activity were investigated by SEM, fluorescent, and digital image analysis. This peptide fraction had high inhibitory effects in surface and deep inoculations of E. coli of 1990.00 and 136.13 mm2/mgPr/µMol, respectively, in the samples. Thus, it would be effective in the treatment of infections involving bacterial biofilms and homogenous cells. Various deformations of the bacteria and inhibition of its metabolism were discovered and illustrated. The data on the mechanisms of impact of the peptides permitted the formulation of an algorithm for the treatment of infections depending on the phase of their development. The decrease in the therapeutic concentrations will be more sparing to the environment and will lead to a decrease in the cost of the treatment.

摘要

从玉米蜗牛黏液中分离出的肽可能成为抗病原菌抗生素的原型。有关作用机制、有效浓度和应用方法的信息是治疗、财务和生态调控以及整体医疗方法的重要工具。使用Autoflex™ III通过基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF-TOF）对分子量小于10 kDa的肽组分进行了分析。使用了大肠杆菌NBIMCC 8785菌株（18小时和48小时培养物）。通过扫描电子显微镜（SEM）、荧光和数字图像分析研究了细菌结构和代谢活性的变化。该肽组分在样品中对1990年的大肠杆菌进行表面接种和深层接种时分别具有1990.00和136.13 mm2/mgPr/µMol的高抑制作用。因此，它在治疗涉及细菌生物膜和均匀细胞的感染方面将是有效的。发现并展示了细菌的各种变形及其代谢抑制情况。关于肽的作用机制的数据允许制定一种根据感染发展阶段进行治疗的算法。治疗浓度的降低将对环境更有利，并将导致治疗成本的降低。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/2e59bef0547a/biomedicines-10-00672-g012.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/54e6600f927c/biomedicines-10-00672-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/2eb2ca59e0fe/biomedicines-10-00672-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/2e59bef0547a/biomedicines-10-00672-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/63ca45dda557/biomedicines-10-00672-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/75b3c6000a3b/biomedicines-10-00672-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/a896c65ae8b6/biomedicines-10-00672-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/818594695029/biomedicines-10-00672-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/a5431bc6c7e4/biomedicines-10-00672-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/54e6600f927c/biomedicines-10-00672-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/df90ed2ce29b/biomedicines-10-00672-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/2eb2ca59e0fe/biomedicines-10-00672-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/32666637484a/biomedicines-10-00672-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/f17c8915c773/biomedicines-10-00672-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/b4a7c6092a08/biomedicines-10-00672-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad66/8945727/2e59bef0547a/biomedicines-10-00672-g012.jpg

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