Petrov Lubomir, Kachaunov Mihail, Alexandrova Albena, Tsvetanova Elina, Georgieva Almira, Dolashki Aleksander, Velkova Lyudmila, Dolashka Pavlina
National Sports Academy "Vassil Levski", 23, Acad. Stefan Mladenov Str., Studentski Grad, 1700 Sofia, Bulgaria.
Laboratory of Free Radical Processes, Institute of Neurobiology, Bulgarian Academy of Sciences, 23, Acad. Georgi Bonchev Str., 1113 Sofia, Bulgaria.
Life (Basel). 2022 Jul 22;12(8):1106. doi: 10.3390/life12081106.
Nowadays, an increased interest in natural compounds with preventive or therapeutic potential for various diseases has been observed. Given the involvement of oxidative stress in the pathogenesis of gastric ulcer (GU) and the wide range of bioactive compounds isolated from snails, this study aimed to investigate the protective effect of (Müller, 1774) mucus on ethanol-induced GUs. Male albino mice were divided into Control, Ethanol, Mucus + Ethanol and Mucus + Omeprazole treated groups. The GUs were induced by administration of 96% ethanol (10 mL/kg, per os). One hour before ulcer induction, the mice of Mucus + Ethanol group were pretreated with mucus (20 mg/kg, per os), and the mice of Mucus + Omeprazole group were pretreated with omeprazole (20 mg/kg, per os). Ethanol administration caused grave lesions of gastric mucosa and a significant decrease of glutathione (GSH) and superoxide dismutase (SOD), catalase, and glutathione reductase (GR) activities. In the animals with mucus or omeprazole pre-administration compared to the Ethanol group, the following were observed: only a small number of hemorrhagic fields, significantly reduced GU index with calculated 73% protection by mucus and 78% protection by omeprazole, and significant recovery of mucosal GSH and SOD and GR activities. In addition, the mucus inhibited growth. Thus, the protective effect of mucus on both gastric mucosa and gastric antioxidant potential in ethanol-induced GU model suggests that it may serve as a good tool for prevention of this disease.
如今，人们对具有预防或治疗各种疾病潜力的天然化合物的兴趣日益增加。鉴于氧化应激参与胃溃疡（GU）的发病机制，以及从蜗牛中分离出的多种生物活性化合物，本研究旨在探讨耳萝卜螺（Müller，1774）黏液对乙醇诱导的胃溃疡的保护作用。将雄性白化病小鼠分为对照组、乙醇组、黏液 + 乙醇组和黏液 + 奥美拉唑治疗组。通过口服给予96%乙醇（10 mL/kg）诱导胃溃疡。在诱导溃疡前1小时，黏液 + 乙醇组小鼠经口给予黏液（20 mg/kg）进行预处理，黏液 + 奥美拉唑组小鼠经口给予奥美拉唑（20 mg/kg）进行预处理。乙醇给药导致胃黏膜严重损伤，谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、过氧化氢酶和谷胱甘肽还原酶（GR）活性显著降低。与乙醇组相比，预先给予黏液或奥美拉唑的动物出现以下情况：仅有少量出血区域，胃溃疡指数显著降低，黏液的保护率计算为73%，奥美拉唑的保护率为78%，黏膜GSH、SOD和GR活性显著恢复。此外，黏液抑制了幽门螺杆菌的生长。因此，耳萝卜螺黏液在乙醇诱导的胃溃疡模型中对胃黏膜和胃抗氧化潜力均具有保护作用，这表明它可能是预防这种疾病的良好工具。
