Kirilova Mihaela, Topalova Yana, Velkova Lyudmila, Dolashki Aleksandar, Kaynarov Dimitar, Daskalova Elmira, Zheleva Nellie
Faculty of Biology, Sofia University, 8 Dragan Tzankov blvd., 1164 Sofia, Bulgaria.
Center of Competence "Clean Technologies for Sustainable Environment-Water, Waste, Energy for Circular Economy", 1000 Sofia, Bulgaria.
Pharmaceuticals (Basel). 2024 Jan 3;17(1):68. doi: 10.3390/ph17010068.
Natural products and especially those from marine organisms are being intensively explored as an alternative to synthetic antibiotics. However, the exact mechanisms of their action are not yet well understood. The molecular masses of components in the hemolymph fraction with MW 50-100 kDa from were determined using ImageQuant™ TL v8.2.0 software based on electrophoretic analysis. Mainly, three types of compounds with antibacterial potential were identified, namely proteins with MW at 50.230 kDa, 62.100 kDa and 93.088 kDa that were homologous to peroxidase-like protein, aplicyanin A and L-amino acid oxidase and functional units with MW 50 kDa from hemocyanin. Data for their antibacterial effect on NBIMCC 8785 were obtained by CTC/DAPI-based fluorescent analysis (analysis based on the use of a functional fluorescence probe). The fluorescent analyses demonstrated that a 50% concentration of the fraction with MW 50-100 kDa was able to eliminate 99% of the live bacteria. The antimicrobial effect was detectable even at a 1% concentration of the active compounds. The bacteria in this case had reduced metabolic activity and a 24% decreased size. The fraction had superior action compared with another mollusc product-snail slime-which killed 60% of the NBIMCC 8785 cells at a 50% concentration and had no effect at a 1% concentration. The obtained results demonstrate the high potential of the fraction with MW 50-100 kDa from to eliminate and suppress the development of NBIMCC 8785 bacteria and could be applied as an appropriate component of therapeutics with the potential to replace antibiotics to avoid the development of antibiotic resistance.
天然产物，尤其是来自海洋生物的天然产物，正作为合成抗生素的替代品而被深入研究。然而，其确切的作用机制尚未完全明确。基于电泳分析，使用ImageQuant™ TL v8.2.0软件测定了来自[具体生物]的分子量在50 - 100 kDa的血淋巴组分中各成分的分子量。主要鉴定出了三种具有抗菌潜力的化合物，即分子量分别为50.230 kDa、62.100 kDa和93.088 kDa的蛋白质，它们分别与过氧化物酶样蛋白、海兔紫素A和L - 氨基酸氧化酶同源，以及来自血蓝蛋白的分子量为50 kDa的功能单元。通过基于CTC/DAPI的荧光分析（基于使用功能性荧光探针的分析）获得了它们对NBIMCC 8785的抗菌效果数据。荧光分析表明，50%浓度的分子量为50 - 100 kDa的组分能够杀灭99%的活菌。即使在活性化合物浓度为1%时也能检测到抗菌效果。在这种情况下，细菌的代谢活性降低，大小减小了24%。与另一种软体动物产品——蜗牛黏液相比，该组分具有更优异的作用，蜗牛黏液在50%浓度时能杀死60%的NBIMCC 8785细胞，而在1%浓度时则没有效果。所得结果表明，来自[具体生物]的分子量为50 - 100 kDa的组分具有消除和抑制NBIMCC 8785细菌生长的巨大潜力，可作为治疗药物的合适成分，有可能替代抗生素以避免抗生素耐药性的产生。
