CF-Clinical Trials Unit, Center for Medical Research, Medical University of Graz, 8036 Graz, Austria.
Division of Endocrinology and Diabetology, Department of Internal Medicine, Medical University of Graz, 8036 Graz, Austria.
Biomolecules. 2022 Mar 18;12(3):466. doi: 10.3390/biom12030466.
Methods for glucagon analysis suffered in the past from lack of specificity and a narrow sensitivity range, which has led to inaccurate results and to the suggestion that type 1 diabetes (T1D) and type 2 diabetes (T2D) patients have elevated fasting glucagon levels. However, the availability of more specific and more sensitive methods to detect intact glucagon has shown that actual glucagon levels are lower than previously assumed. This study aimed to characterize fasting plasma glucagon levels in healthy individuals and T1D and T2D patients with two different glucagon assays. The study included 20 healthy individuals, 20 T1D and 20 T2D patients. Blood was collected under fasting conditions. A double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) and a conventional radioimmunoassay (RIA) were used. A significant difference in fasting glucagon levels between healthy individuals and T2D was observed by ELISA, but not by RIA. ELISA also yielded lower glucagon levels in healthy individuals than in T1D and T2D patients which RIA did not. RIA produced significantly ( = 0.0001) higher overall median glucagon values than ELISA in a pooled analysis. These results underline the notion that the choice of selective laboratory methods is highly relevant for mechanistic endocrine research.
方法的胰高血糖素分析过去从缺乏特异性和狭窄的灵敏度范围,这导致了不准确的结果,并建议 1 型糖尿病 (T1D) 和 2 型糖尿病 (T2D) 患者空腹胰高血糖素水平升高。然而,更特异和更敏感的方法来检测完整的胰高血糖素的可用性表明实际的胰高血糖素水平低于先前假设。本研究旨在描述健康个体和 T1D 和 T2D 患者空腹血浆胰高血糖素水平用两种不同的胰高血糖素检测。这项研究包括 20 名健康个体、20 名 T1D 和 20 名 T2D 患者。在空腹条件下采集血液。采用双抗体夹心酶联免疫吸附试验 (ELISA) 和常规放射免疫分析 (RIA)。ELISA 观察到空腹胰高血糖素水平在健康个体和 T2D 之间存在显著差异，但 RIA 则没有。ELISA 还产生了比 RIA 更低的健康个体的胰高血糖素水平，而 RIA 则没有。在 pooled 分析中，RIA 产生的总体中位数胰高血糖素值显著高于 ELISA( = 0.0001)。这些结果强调了选择选择性实验室方法对于机制内分泌研究非常重要。
