Łabuz Grzegorz, Zielińska Agnieszka, Kessler Lucy J, Rayamajhi Asu, Komar Katarzyna, Khoramnia Ramin, Auffarth Gerd U
The David J Apple Center for Vision Research, Department of Ophthalmology, University Hospital Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany.
Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University in Toruń, Grudziądzka 5, 87-100 Toruń, Poland.
Diagnostics (Basel). 2022 Mar 21;12(3):760. doi: 10.3390/diagnostics12030760.
The recently introduced term “two-photon vision” relates to the visual perception resulting from a simultaneous absorption of two photons by photoreceptors. In this study, we determined two-photon retinal sensitivity in age-related macular degeneration (AMD) and compared it that in normal aging. Microperimetry was performed with visible (white) light and infrared (IR) light, which was perceived as green in the two-photon stimulation. In total, 45 subjects were included with one (better) eye studied. Furthermore, best-corrected visual acuity (VA) and ocular straylight were assessed. AMD resulted in decreased median (interquartile range) logMAR VA, i.e., 0.15 (0.05; 0.24), which in normal eyes was −0.02 (−0.06; 0.02). The two groups showed comparable straylight levels. Sensitivity to IR light was significantly lower in the AMD group (p < 0.001): 8.3 (7.4, 9.3) dB than in controls 10.7 (9.7, 11.2) dB. AMD also significantly affected visible light sensitivity (p < 0.001): 14.0 (11.0; 15.5) dB vs. 18.0 (16.3; 18.9) dB. Notably, the two-photon approach yielded a lower data spread. In conclusion, AMD considerably impairs retinal sensitivity measured in the single- and two-photon realm. However, two-photon-vision microperimetry may improve the testing accuracy and offer an additional diagnostic parameter (beyond VA measurements) for retinal function assessment.
最近引入的术语“双光子视觉”与光感受器同时吸收两个光子所产生的视觉感知有关。在本研究中，我们测定了年龄相关性黄斑变性（AMD）患者的双光子视网膜敏感度，并将其与正常衰老者进行比较。使用可见光（白光）和红外光（IR）进行微视野检查，在双光子刺激下，红外光被感知为绿色。总共纳入了45名受试者，研究其一只（较好的）眼睛。此外，还评估了最佳矫正视力（VA）和眼内杂散光。AMD导致logMAR VA的中位数（四分位间距）降低，即0.15（0.05；0.24），而正常眼睛为-0.02（-0.06；0.02）。两组的杂散光水平相当。AMD组对红外光的敏感度显著低于对照组（p<0.001）：分别为8.3（7.4，9.3）dB和10.7（9.7，11.2）dB。AMD对可见光敏感度也有显著影响（p<0.001）：分别为14.0（11.0；15.5）dB和18.0（16.3；18.9）dB。值得注意的是，双光子方法的数据离散度较低。总之，AMD严重损害了在单光子和双光子领域测得的视网膜敏感度。然而，双光子视觉微视野检查可能会提高测试准确性，并为视网膜功能评估提供一个额外的诊断参数（除VA测量外）。
