人类腺病毒 4 型病毒相关 RNA I 的结构洞察。

Structural Insights into Human Adenovirus Type 4 Virus-Associated RNA I.

机构信息

Department of Medical Biochemistry and Microbiology, Uppsala University, BMC Box 582, Husargatan 3, SE-751 23 Uppsala, Sweden.

Department of Laboratory Medicine, Translational Research Center Karolinska (TRACK), Karolinska Institutet, Huddinge, SE-141 86 Stockholm, Sweden.

出版信息

Int J Mol Sci. 2022 Mar 13;23(6):3103. doi: 10.3390/ijms23063103.

DOI:10.3390/ijms23063103

PMID:35328524

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8949993/

Abstract

RNA molecules can adopt specific RNA triplex structures to execute critical biological functions. Human adenoviruses (HAdVs) are abundant pathogens encoding the essential, noncoding virus-associated RNA I (VA RNAI). Here, we employ a triplex-specific probing assay, based on the intercalating and cleaving agent benzoquinoquinoxaline 1, 10-phenanthroline (BQQ-OP), to unravel a potential RNA triplex formation in VA RNAI. The BQQ-OP cleavage of the pathogenic HAdV type 4 (HAdV-4) VA RNAI indicates that a potential triplex is formed involving the highly conserved stem 4 of the central domain and side stem 7. Further, the integrity of the HAdV-4 VA RNAI side stem 7 contributes to a potential triplex formation in vitro and virus growth in vivo. Collectively, we propose that the HAdV-4 VA RNAI can potentially form a biologically relevant triplex structure.

摘要

RNA 分子可以采用特定的 RNA 三聚体结构来执行关键的生物学功能。人类腺病毒（HAdV）是大量存在的病原体，其编码必需的非编码病毒相关 RNA I（VA RNAI）。在这里，我们利用基于嵌入和切割剂苯并喹喔啉 1,10-菲咯啉（BQQ-OP）的三聚体特异性探测测定法，揭示 VA RNAI 中可能存在的 RNA 三聚体形成。致病型 HAdV 型 4（HAdV-4）VA RNAI 的 BQQ-OP 切割表明，形成了一种涉及中央结构域高度保守的茎 4 和侧茎 7 的潜在三聚体。此外，HAdV-4 VA RNAI 侧茎 7 的完整性有助于体外潜在三聚体的形成和体内病毒的生长。总之，我们提出 HAdV-4 VA RNAI 可能形成具有生物学意义的三聚体结构。