Institute for Bioprocessing and Analytical Measurement Techniques e.V. (iba) Rosenhof, 37308 Heilbad Heiligenstadt, Germany.
Multiphoton Optics GmbH, Friedrich-Bergius-Ring 15, 97076 Wuerzburg, Germany.
Int J Mol Sci. 2022 Mar 14;23(6):3115. doi: 10.3390/ijms23063115.
An aging population and injury-related damage of the bone substance lead to an increasing need of innovative materials for the regeneration of osteochondral defects. Biodegradable polymers form the basis for suitable artificial implants intended for bone replacement or bone augmentation. The great advantage of these structures is the site-specific implant design, which leads to a considerable improvement in patient outcomes and significantly reduced post-operative regeneration times. Thus, biomechanical and biochemical parameters as well as the rate of degradation can be set by the selection of the polymer system and the processing technology. Within this study, we developed a polymer platform based on the amino acid Alanine and ε-Caprolacton for use as raw material for osteochondral implants. The biomechanical and degradation properties of these Poly-(Alanine-co-ε-Caprolacton)-Methacrylate (ACM) copolymers can be adjusted by changing the ratio of the monomers. Fabrication of artificial structures for musculo-skeletal tissue engineering was done by Two-Photon-Polymerization (2PP), which represents an innovative technique for generating defined scaffolds with tailor-made mechanical and structural properties. Here we show the synthesis, physicochemical characterization, as well as first results for structuring ACM using 2PP technology. The data demonstrate the high potential of ACM copolymers as precursors for the fabrication of biomimetic implants for bone-cartilage reconstruction.
人口老龄化和与损伤相关的骨物质损害导致对用于骨软骨缺损再生的创新材料的需求不断增加。可生物降解聚合物是用于骨置换或骨增强的合适人工植入物的基础。这些结构的巨大优势在于特定部位的植入物设计，这导致患者的预后得到了极大的改善，并且术后的再生时间显著缩短。因此，可以通过选择聚合物体系和加工技术来设置生物力学和生化参数以及降解速率。在这项研究中，我们开发了一种基于氨基酸丙氨酸和 ε-己内酯的聚合物平台，用作骨软骨植入物的原材料。通过改变单体的比例，可以调整这些聚（丙氨酸-共-ε-己内酯）-甲基丙烯酸酯（ACM）共聚物的生物力学和降解性能。通过双光子聚合（2PP）来制造用于肌肉骨骼组织工程的人工结构，这是一种用于生成具有定制机械和结构性能的定义支架的创新技术。在这里，我们展示了 ACM 的合成、物理化学特性，以及使用 2PP 技术对其进行结构设计的初步结果。这些数据表明 ACM 共聚物作为用于骨软骨重建的仿生植入物制造的前体具有很高的潜力。
