Graduate Program of Biotechnology and Pharmaceutical Industries, National Taiwan Normal University, Taipei 106, Taiwan.
Traditional Chinese Medicine Department, En Chu Kong Hospital, New Taipei City 237, Taiwan.
Int J Mol Sci. 2022 Mar 15;23(6):3165. doi: 10.3390/ijms23063165.
Type 1 diabetes stem-cell-based therapy is one of the best therapeutic approaches for pancreatic damage treatment due to stem cell tissue regeneration. Epigallocatechin gallate (EGCG) is one of the active components found in green tea. Experimental results suggest that EGCG shows beneficial effects on cell protection. This study explores whether a better pancreatic regeneration therapeutic effect could be found in mesenchymal stem cells pretreated with EGCG compared to stem cells without EGCG pretreatment. A cell model confirmed that adipose-derived stem cells (ADSC) incubated with EGCG increase cell viability under high-glucose (HG) stress. This is due to survival marker p-Akt expression. In an animal model, type 1 diabetes induced the activation of several pathological signals, including islet size reduction, extracellular fibrotic collagen deposition, oxidative stress elevation, survival pathway suppression, apoptosis signaling induction, and Sirt1 antioxidant pathway downregulation. Ordinary ADSC transplantation slightly improved the above pathological signals. Further, EGCG-pretreated ADSC transplantation significantly improved the above pathological conditions. Taken together, EGCG-pretreated ADSCs show clinical potential in the treatment of patients with type 1 diabetes through the regeneration of damaged pancreatic tissues.
1 型糖尿病基于干细胞的治疗是治疗胰腺损伤的最佳治疗方法之一，因为干细胞组织再生。表没食子儿茶素没食子酸酯（EGCG）是绿茶中发现的一种活性成分。实验结果表明，EGCG 对细胞保护具有有益作用。本研究探讨了与未经 EGCG 预处理的干细胞相比，用 EGCG 预处理间充质干细胞是否能找到更好的胰腺再生治疗效果。细胞模型证实，在高糖（HG）应激下，用 EGCG 孵育的脂肪来源干细胞（ADSC）可增加细胞活力。这是由于存活标志物 p-Akt 的表达。在动物模型中，1 型糖尿病诱导了几种病理信号的激活，包括胰岛缩小、细胞外纤维胶原沉积、氧化应激升高、存活途径抑制、细胞凋亡信号诱导以及 Sirt1 抗氧化途径下调。普通 ADSC 移植仅轻微改善了上述病理信号。此外，用 EGCG 预处理的 ADSC 移植可显著改善上述病理状况。综上所述，EGCG 预处理的 ADSC 通过损伤胰腺组织的再生显示出在治疗 1 型糖尿病患者方面的临床潜力。
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