Voicu Sorina Nicoleta, Gheran Cecilia Virginia, Balta Cornel, Hermenean Anca, Callewaert Maité, Chuburu Françoise, Dinischiotu Anca
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 050095 Bucharest, Romania.
Department of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, 310414 Arad, Romania.
Polymers (Basel). 2024 Apr 11;16(8):1064. doi: 10.3390/polym16081064.
The aim of this study was the investigation of biochemical and histological changes induced in different tissues, as a result of the subcutaneous administration of Gd nanohydrogels (GdDOTA⸦CS-TPP/HA) in a CD-1 mouse strain. The nanohydrogels were obtained by encapsulating contrast agents (GdDOTA) in a biocompatible polymer matrix composed of chitosan (CS) and hyaluronic acid (HA) through the ionic gelation process. The effects of Gd nanohydrogels on the redox status were evaluated by measuring specific activities of the antioxidant enzymes catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD), as well as oxidative stress markers, such as reduced glutathione (GSH), malondialdehyde (MDA), advanced oxidation protein products (AOPP), and protein-reactive carbonyl groups (PRCG), in the liver, kidney, and heart tissues. The nitrosylated proteins expression were analyzed with Western Blot and the serum biochemical markers were measured with spectrophotometric methods. Also, a histological analysis of CD-1 mouse tissues was investigated. These results indicated that Gd nanohydrogels could potentially be an alternative to current MRI contrast agents thanks to their low toxicity in vivo.
本研究的目的是调查在CD-1小鼠品系中皮下注射钆纳米水凝胶（GdDOTA⸦CS-TPP/HA）后不同组织中诱导的生化和组织学变化。通过离子凝胶化过程将造影剂（GdDOTA）包裹在由壳聚糖（CS）和透明质酸（HA）组成的生物相容性聚合物基质中，从而获得纳米水凝胶。通过测量抗氧化酶过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）的比活性，以及氧化应激标志物，如肝脏、肾脏和心脏组织中的还原型谷胱甘肽（GSH）、丙二醛（MDA）、晚期氧化蛋白产物（AOPP）和蛋白质反应性羰基基团（PRCG），评估钆纳米水凝胶对氧化还原状态的影响。用蛋白质免疫印迹法分析亚硝基化蛋白质的表达，并用分光光度法测量血清生化标志物。此外，还对CD-1小鼠组织进行了组织学分析。这些结果表明，钆纳米水凝胶由于其在体内的低毒性，可能成为当前磁共振成像造影剂的替代品。
