Tsilo Phakamani H, Basson Albertus K, Ntombela Zuzingcebo G, Maliehe Tsolanku S, Pullabhotla V S R Rajasekhar
Department of Biochemistry and Microbiology, Faculty of Science, Agriculture and Engineering, University of Zululand, P/Bag X1001, KwaDlangezwa 3886, South Africa.
Department of Chemistry, Faculty of Sciences, Agriculture and Engineering, University of Zululand, P/Bag X1001, KwaDlangezwa 3886, South Africa.
Int J Environ Res Public Health. 2022 Mar 8;19(6):3148. doi: 10.3390/ijerph19063148.
A variety of flocculants have been used to aggregate colloidal substances. However, recently, owing to the adverse effects and high costs of conventional flocculants, natural flocculants such as microbial flocculants are gaining attention. The aim of the study was to produce and characterize a bioflocculant from MH545928.1 and apply it in wastewater treatment. A mixture of butanol and chloroform (5:2 /) was used to extract the bioflocculant. Phenol-sulphuric acid, Bradford and Carbazole assays were utilized for the identification of carbohydrates, proteins and uronic acid, respectively. Scanning electron microscopy (SEM) and elemental detector were employed to determine the surface morphology and elemental compositions. The removal efficiencies were 73%, 49% and 47% for BOD, COD and P, respectively. The bioflocculant (2.836 g/L) obtained showed the presence of carbohydrates (69%), protein (11%) and uronic acid (16%). The bioflocculant displayed a cumulus-like structure and the elemental composition of C (16.92%), N (1.03%), O (43:76%), Na (0.18%), Mg (0.40%), Al (0.80%), P (14.44%), S (1.48%), Cl (0.31%), K (0.34%) and Ca (20.35). It showed the removal efficiencies of 43% (COD), 64% (BOD), 73% (P) and 50% (N) in coal mine wastewater. This bioflocculant is potentially viable to be used in wastewater treatment.
多种絮凝剂已被用于凝聚胶体物质。然而，近来由于传统絮凝剂的副作用和高成本，诸如微生物絮凝剂等天然絮凝剂正受到关注。该研究的目的是从MH545928.1中制备一种生物絮凝剂并对其进行表征，然后将其应用于废水处理。使用丁醇和氯仿的混合物（5:2 /）来提取生物絮凝剂。分别采用苯酚 - 硫酸法、考马斯亮蓝法和咔唑法来鉴定碳水化合物、蛋白质和糖醛酸。利用扫描电子显微镜（SEM）和元素探测器来确定表面形态和元素组成。对生化需氧量（BOD）、化学需氧量（COD）和磷（P）的去除效率分别为73%、49%和47%。所获得的生物絮凝剂（2.836 g/L）含有碳水化合物（69%）、蛋白质（11%）和糖醛酸（16%）。该生物絮凝剂呈现出积云状结构，其元素组成为碳（C，16.92%）、氮（N，1.03%）、氧（O，43.76%）、钠（Na，0.18%）、镁（Mg，0.40%）、铝（Al，0.80%）、磷（P，14.44%）、硫（S，1.48%）、氯（Cl，0.31%）、钾（K，0.34%）和钙（Ca，20.35%）。它对煤矿废水的去除效率为化学需氧量（COD）43%、生化需氧量（BOD）64%、磷（P）73%和氮（N）50%。这种生物絮凝剂在废水处理中具有潜在的应用可行性。
