Sibisi Minenhle Pd, Basson Albertus K, Ntombela Zuzingcebo G, Pullabhotla Rajasekhar V S R
Department of Biochemistry and Microbiology, Faculty of Science, Agriculture and Engineering, University of Zululand, South Africa.
Department of Chemistry, Faculty of Science, Agriculture and Engineering, University of Zululand, South Africa.
Biotechnol Notes. 2025 Feb 22;6:89-99. doi: 10.1016/j.biotno.2025.02.002. eCollection 2025.
Nanotechnology is being used to solve a variety of environmental issues, including wastewater treatment. In the present study, a rapid eco-friendly method was applied to biosynthesize and optimize copper nanoparticles (CuNPs) from . The CuNPs characteristics were identified using X-ray diffractometer (XRD), scanning electron microscope (SEM), Fourier Transform infrared (FT-IR), Transmission electron microscope (TEM), Thermogravimetric analysis (TGA) and UV-Vis spectroscope (UV-Vis). To determine the maximum metabolic yield, the optimum dosage size, pH, temperature, salinity and cations were evaluated. The antibacterial activity of the samples against Gram-negative and Gram-positive isolates was assessed using the Kirby-Bauer Disk Diffusion Test. 28.3 nm was the average crystallite size of CuNPs revealed through XRD analysis. The SEM and TEM analysis depicted the CuNPs to be agglomerated in various sizes and forms. Elements such as Carbon (25.23 % wt), Cu (23.37 % Wt) and Oxygen (20.13 % Wt) were found in CuNPs. The nanoparticles had functional groups and a Cu-O bond at 559 cm . The CuNPs retained 70 % of its weight whereas the bioflocculant retained only 50 % when heated at a range of 100 °C-900 °C. The samples exhibited a UV-Vis spectra between 250 and 300 nm, at a range of 200-1400 nm. The flocculating effeciency of CuNPs was optimal at 0.2 mg/mL (92 %) and cation independent (92 %). pH 7 was the peak maximum as 98 % of the flocculating activity was obtained. The CuNPs were thermally stable than the bioflocculant as over 80 % of its flocculating activity was retained even at high temperatures (121 °C). The CuNPs were not affected by the increase in NaCl concentration with the highest NaCl concentration (35 g/L) having the highest flocculating activity of 90 %. CuNPs exhibited antimicrobial activity against both bacterial strains, with greater susceptibility observed in as compared to the bioflocculant. Thus, CuNPs have a potential to be applied in wastewater treatment to replace traditional flocculants.
纳米技术正被用于解决各种环境问题，包括废水处理。在本研究中，采用了一种快速环保的方法从……生物合成并优化铜纳米颗粒（CuNPs）。使用X射线衍射仪（XRD）、扫描电子显微镜（SEM）、傅里叶变换红外光谱仪（FT-IR）、透射电子显微镜（TEM）、热重分析（TGA）和紫外可见光谱仪（UV-Vis）对CuNPs的特性进行了鉴定。为了确定最大代谢产量，评估了最佳剂量大小、pH值、温度、盐度和阳离子。使用 Kirby-Bauer 纸片扩散法评估样品对革兰氏阴性菌和革兰氏阳性菌分离株的抗菌活性。XRD分析显示CuNPs的平均微晶尺寸为28.3 nm。SEM和TEM分析表明CuNPs以各种大小和形式团聚。在CuNPs中发现了碳（25.23%重量）、铜（23.37%重量）和氧（20.13%重量）等元素。纳米颗粒具有官能团，在559 cm 处有Cu-O键。当在100°C至900°C范围内加热时，CuNPs保留了其70%的重量，而生物絮凝剂仅保留了50%。样品在200 - 1400 nm范围内的250至300 nm处呈现紫外可见光谱。CuNPs的絮凝效率在0.2 mg/mL时最佳（92%），且与阳离子无关（92%）。pH值为7时达到峰值，絮凝活性达到98%。CuNPs比生物絮凝剂热稳定性更好，即使在高温（121°C）下仍保留超过80%的絮凝活性。CuNPs不受NaCl浓度增加的影响，最高NaCl浓度（35 g/L）时絮凝活性最高，为90%。CuNPs对两种细菌菌株均表现出抗菌活性，与生物絮凝剂相比，[此处原文似乎缺失部分内容]对其更敏感。因此，CuNPs有潜力应用于废水处理以替代传统絮凝剂。
