Pickard C E, Stewart A D, Hartley R, Lucock M D
Ann Clin Biochem. 1986 Jul;23 ( Pt 4):440-6. doi: 10.1177/000456328602300410.
A simple HPLC method for the determination of caffeine and theophylline in plasma is described. Separation of theobromine, paraxanthine, theophylline, beta-hydroxyethyltheophylline and caffeine is obtained using a mobile phase of 1% acetic acid/methanol (83:17, v/v) and a Waters Associates NOVA-PAK C18 column protected by a Guard-PAK precolumn module containing a Guard-PAK CN cartridge. Rapid sample preparation is achieved by solid-phase extraction columns (Bond-Elut C18, 1 mL capacity) which provide excellent recovery values for both drugs. The cost per sample using this approach can be minimised by column regeneration and re-use. Results obtained for theophylline are in good agreement with values determined by other techniques.
描述了一种用于测定血浆中咖啡因和茶碱的简单高效液相色谱法。使用1%乙酸/甲醇（83:17，v/v）的流动相和由包含Guard-PAK CN柱芯的Guard-PAK预柱模块保护的Waters Associates NOVA-PAK C18柱，可实现可可碱、副黄嘌呤、茶碱、β-羟乙基茶碱和咖啡因的分离。通过固相萃取柱（Bond-Elut C18，1 mL容量）可实现快速样品制备，该柱对两种药物均提供出色的回收率。通过柱再生和重复使用，可将使用此方法的每个样品成本降至最低。获得的茶碱结果与通过其他技术测定的值高度一致。
