Department of Rehabilitation and Orthopaedics, Medical University of Lublin, 20-059 Lublin, Poland.
Department of Orthopaedics and Traumatology, Regional Hospital in Chełm, 22-100 Chełm, Poland.
Int J Environ Res Public Health. 2022 Mar 16;19(6):3530. doi: 10.3390/ijerph19063530.
The aim of the study was to evaluate the levels of selected MMPs (matrix metalloproteinases) and osteocalcin in the serum and synovial fluid of obese women with osteoarthritis and their correlations with clinical status. The studied group consisted of 39 overweight females undergoing primary total knee arthroplasty due to osteoarthritis (OA). The staging of knee OA was evaluated according to the Ahlbӓck and Kellgren-Lawrence scores. Synovial fluid and peripheral blood samples were obtained. The levels of selected MMPs and osteocalcin were assessed using commercial ELISA kits. The mean value of MMP3 was significantly higher in patients with more advanced disease in both serum ( = 0.0067) and synovial fluid ( = 0.0328). The pro-MMP13 level tended to be higher in synovial fluid in the case of more advanced stages ( = 0.0882), with no tendency regarding the serum level ( = 0.9595). The synovial level of pro-MMP1 was significantly correlated with the synovial concentration of MMP9 and MMP3. The synovial level of MMP9 also showed a significant correlation with the synovial level of MMP3 and pro-MMP13. Furthermore, it was found that the serum level of MMP3 was significantly correlated with the synovial pro-MMP13 level. A correlation between the osteocalcin level in serum and its synovial level was determined. The serum MMP9 level showed a significant correlation with BMI, whereas the synovial MMP9 level was notably correlated with age. Our results showed that the levels of MMP3, MMP9, and pro-MMP13 increased in more advanced radiological stages of OA, indicating the underlying inflammatory process of OA.
本研究旨在评估肥胖女性骨性关节炎患者血清和滑液中选定的基质金属蛋白酶（MMPs）和骨钙素水平及其与临床状况的相关性。研究组包括 39 名因骨性关节炎（OA）行初次全膝关节置换术的超重女性。OA 的膝关节分期根据 Ahlbӓck 和 Kellgren-Lawrence 评分进行评估。采集滑液和外周血样本。使用商业 ELISA 试剂盒评估选定的 MMPs 和骨钙素的水平。在血清（ = 0.0067）和滑液（ = 0.0328）中，疾病进展更严重的患者 MMP3 的平均值明显更高。在更严重的阶段，MMP13 的前体水平在滑液中趋于更高（ = 0.0882），而血清水平没有趋势（ = 0.9595）。MMP1 前体在滑液中的水平与 MMP9 和 MMP3 在滑液中的浓度显著相关。MMP9 的滑液水平与 MMP3 和 MMP13 的滑液水平也呈显著相关性。此外，还发现血清 MMP3 水平与滑液中 pro-MMP13 水平显著相关。确定了血清中骨钙素水平与其滑液水平之间的相关性。血清 MMP9 水平与 BMI 呈显著相关性，而滑液 MMP9 水平与年龄显著相关。我们的研究结果表明，MMP3、MMP9 和 pro-MMP13 的水平在 OA 的更严重的放射学分期中增加，表明 OA 存在潜在的炎症过程。
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