This study aimed to analyze if chronic low back pain (LBP) and lumbar disc herniation induce biomechanics, flexibility, body balance, physical activity, and muscular function alterations compared to a similar asymptomatic cohort. Fifty male volunteers (n = 25 with chronic LBP and lumbar disc herniation and n = 25 pain-free subjects) were enrolled. Range of motion (internal and external hip rotation, ankle dorsiflexion, and active straight leg raise, ASLR), trunk flexibility (finger−floor distance), body balance (Y-balance test) and muscle function (Biering−Sorensen test, prone and lateral bridges) outcomes were assessed. Comparative analyses between sides and group were conducted. Results: Patients showed greater weight and BMI compared with controls (p < 0.05). None of the outcomes bilaterally assessed showed side-to-side differences for pain-free participants (all, p > 0.05) or LBP patients (all, p > 0.05). Regarding the differences between groups, LBP patients showed limited internal hip rotation (p < 0.001), finger−floor distance (p < 0.001), body balance (p < 0.001), and muscle endurance (planks p < 0.001; Biering−Sorensen test p < 0.05). External hip rotation, ASLR, and ankle dorsiflexion range of movement were comparable in both groups (p > 0.05). The sample of pain-free amateur athletes showed greater range of movement for internal hip rotation, lower finger−floor distance, better body balance, and muscle function. However, the external hip rotation, ankle dorsiflexion, and ASLR tests showed no difference between cases and controls.