Chen Tingting, Wu Xia, Dai Yunyun, Yin Xianhui, Zhao Zhibo, Zhang Zhuzhu, Li Wenzhi, He Linan, Long Youhua
Research Center for Engineering Technology of Kiwifruit, Institute of Crop Protection, College of Agriculture, Guizhou University, Guiyang 550025, China.
Teaching Experimental Field of Guizhou University, Guizhou University, Guiyang 550025, China.
J Fungi (Basel). 2022 Feb 27;8(3):239. doi: 10.3390/jof8030239.
Kiwifruit is a nutritious and economically important fruit that is widely cultivated in China. In 2021, leaf spot disease of kiwifruit was discovered in the main kiwifruit-producing area of Xifeng County, Guizhou Province, China. Leaf spot disease weakens plant photosynthesis and reduces nutrient synthesis, thereby affecting plant growth. We studied the morphological characteristics and performed a combined analysis of , , and genes of a fungus associated with leaf spot disease. The pathogenicity of followed Koch's hypothesis, confirming that this fungus is the cause of kiwifruit leaf spot disease. The sensitivity of seven natural antifungal agents against was measured using the mycelial growth rate method. Honokiol, cinnamaldehyde, and osthol showed good antifungal effects against , with EC values of 18.50, 64.60, and 64.86 μg/mL, respectively. The regression coefficient of cinnamaldehyde was the largest at 2.23, while that of honokiol was the smallest at 0.408. was the most sensitive to cinnamaldehyde.
猕猴桃是一种营养丰富且具有重要经济价值的水果，在中国广泛种植。2021年，在中国贵州省息烽县的主要猕猴桃产区发现了猕猴桃叶斑病。叶斑病会削弱植物的光合作用并减少养分合成，从而影响植物生长。我们研究了与叶斑病相关的一种真菌的形态特征，并对其 、 和 基因进行了联合分析。该菌的致病性符合柯赫氏法则，证实这种真菌是猕猴桃叶斑病的病因。采用菌丝生长速率法测定了七种天然抗真菌剂对该菌的敏感性。厚朴酚、肉桂醛和蛇床子素对该菌表现出良好的抗真菌效果，其EC值分别为18.50、64.60和64.86 μg/mL。肉桂醛的回归系数最大，为2.23，而厚朴酚的回归系数最小，为0.408。该菌对肉桂醛最为敏感。
