York Whitney, Smith M Ryan, Liu Chin-Chi
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, United States.
Front Vet Sci. 2022 Mar 7;9:827350. doi: 10.3389/fvets.2022.827350. eCollection 2022.
A new, portable bedside coagulation monitor (VCM Vet) has provided a user-friendly, cartridge-based method to perform viscoelastic testing. However, the use of native whole blood limits the time to analyze the sample to minutes. The objective of this study is to assess whether citrated whole blood can be utilized with the cartridge-based system and whether the results are comparable to those of native whole blood. A secondary objective is to assess the viability of citrated whole blood results after up to 4 hours of resting.
The study population consisted of 10 healthy mixed breed dogs. Whole blood samples were collected jugular venipuncture. Blood was immediately transferred to the VCM test cartridge for native whole blood control group analysis per the manufacturer's instructions, and the remainder was used to fill two 3.2% sodium citrate vacutainer tubes. Test group analysis was performed on samples from each tube concurrently after a rest period of 30 min (baseline), 2 h, and 4 h. Citrated whole blood samples were recalcified for analysis immediately prior to introduction into the test cartridge. Data was recorded for all reported parameters. Results from the citrate groups were compared to the control group and to the citrated baseline to assess for differences. Overall results were compared using mixed ANOVA models. Where found, specific differences were evaluated using Tukey's test. Within-sample variation was investigated and reported as median (range). A < 0.05 was considered significant.
Samples were obtained for a total of 10 control runs and 20 citrated whole blood runs. Comparison of controls to the citrated test groups revealed significant differences in CT ( < 0.001) and MCF ( < 0.002). There were no significant differences between test groups compared to citrated baselines for any parameter. Selected median coefficients of variation were 6.8% (0-68.8%) for CT, 2.4% (0-19.46%) for alpha angle, 3.2% (0-27.4%) for MCF, and 0% (0-16.3%) for 45-min LY45.
Citrated whole blood samples can be used with the VCM Vet device; however, new reference intervals for use with citrated whole blood will be required. Results using citrated whole blood samples are not significantly different from baseline after up to 4 h of resting.
一种新型便携式床边凝血监测仪（VCM Vet）提供了一种用户友好的、基于试剂盒的方法来进行粘弹性测试。然而，使用天然全血会将样本分析时间限制在几分钟内。本研究的目的是评估枸橼酸盐抗凝血是否可用于基于试剂盒的系统，以及结果是否与天然全血的结果具有可比性。第二个目的是评估枸橼酸盐抗凝血样本在静置长达4小时后的可行性。
研究对象为10只健康的混血犬。通过颈静脉穿刺采集全血样本。血液立即按照制造商的说明转移到VCM测试试剂盒中，用于天然全血对照组分析，其余部分用于填充两个3.2%枸橼酸钠真空采血管。在休息30分钟（基线）、2小时和4小时后，同时对每个采血管中的样本进行测试组分析。在将枸橼酸盐抗凝血样本引入测试试剂盒之前，立即重新钙化以进行分析。记录所有报告参数的数据。将枸橼酸盐组的结果与对照组以及枸橼酸盐基线进行比较，以评估差异。使用混合方差分析模型比较总体结果。若发现差异，使用Tukey检验评估具体差异。研究并报告样本内变异，以中位数（范围）表示。P < 0.05被认为具有显著性。
共进行了10次对照运行和20次枸橼酸盐抗凝血运行的样本采集。对照组与枸橼酸盐测试组的比较显示，凝血时间（CT）（P < 0.001）和最大凝块硬度（MCF）（P < 0.002）存在显著差异。与枸橼酸盐基线相比，测试组在任何参数上均无显著差异。选定的变异系数中位数为：CT为6.8%（0 - 68.8%），α角为2.4%（0 - 19.46%），MCF为3.2%（0 - 27.4%），45分钟纤维蛋白溶解率（LY45）为0%（0 - 16.3%）。
枸橼酸盐抗凝血样本可用于VCM Vet设备；然而，需要新的与枸橼酸盐抗凝血相关的参考区间。使用枸橼酸盐抗凝血样本的结果在静置长达4小时后与基线无显著差异。
