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评估 [F]四氟硼酸作为钠碘同向转运体转染的 A549 细胞系和内源性 NIS 表达细胞系 MKN45 和 K1 中的潜在正电子发射断层扫描成像剂。

Evaluation of [F]tetrafluoroborate as a Potential PET Imaging Agent in a Sodium Iodide Symporter-Transfected Cell Line A549 and Endogenous NIS-Expressing Cell Lines MKN45 and K1.

机构信息

Department of Radiation Medicine, School of Basic Medicine, Chongqing Medical University, Chongqing, China.

出版信息

Mol Imaging. 2022 Feb 27;2022:2679260. doi: 10.1155/2022/2679260. eCollection 2022.

DOI:10.1155/2022/2679260
PMID:35330799
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8923191/
Abstract

[F]tetrafluoroborate (TFB) has been introduced as the F-labeled PET imaging probe for the human sodium iodide symporter (NIS). Noninvasive NIS imaging using [F]TFB has received much interest in recent years for evaluating various NIS-expressing tumors. Cancers are a global concern with enormous implications; therefore, improving diagnostic methods for accurate detection of cancer is extremely important. Our aim was to investigate the PET imaging capabilities of [F]TFB in NIS-transfected lung cell line A549 and endogenous NIS-expressing tumor cells, such as thyroid cancer K1 and gastric cancer MKN45, and broaden its application in the medical field. Western blot and flow cytometry were used to assess the NIS expression level. Radioactivity counts of [F]TFB, , in the three tumor cells were substantially higher than those in the KI inhibition group in the uptake experiment. PET imaging clearly delineated the three tumors based on the specific accumulation of [F]TFB in a mouse model. biodistribution investigation showed high [F]TFB absorption in the tumor location, which was consistent with the PET imaging results. These results support the use of NIS-transfected lung cell line A549 and NIS-expressing tumor cells MKN45 and K1, to investigate probing capabilities of [F]TFB. We also demonstrate, for the first time, the feasibility of [F]TFB in diagnosing stomach cancer. In conclusion, this study illustrates the promising future of [F]TFB for tumor diagnosis and NIS reporter imaging.

摘要

四氟硼酸（TFB）已被引入作为用于人类钠碘转运体（NIS）的 F 标记的正电子发射断层扫描（PET）成像探针。近年来，使用 [F]TFB 进行非侵入性 NIS 成像已引起广泛关注，可用于评估各种表达 NIS 的肿瘤。癌症是一个全球性问题，具有巨大影响；因此，改进用于准确检测癌症的诊断方法非常重要。我们的目的是研究 [F]TFB 在转染 NIS 的肺细胞系 A549 和内源性表达 NIS 的肿瘤细胞（如甲状腺癌 K1 和胃癌 MKN45）中的 PET 成像能力，并拓宽其在医学领域的应用。使用 Western blot 和流式细胞术评估 NIS 的表达水平。摄取实验中，三种肿瘤细胞中 [F]TFB 的放射性计数明显高于 KI 抑制组。在小鼠模型中，基于 [F]TFB 的特异性积累，PET 成像清楚地区分了这三种肿瘤。生物分布研究表明，肿瘤部位的 [F]TFB 吸收很高，与 PET 成像结果一致。这些结果支持使用转染 NIS 的肺细胞系 A549 和表达 NIS 的肿瘤细胞 MKN45 和 K1 来研究 [F]TFB 的探测能力。我们还首次证明了 [F]TFB 诊断胃癌的可行性。总之，这项研究表明了 [F]TFB 用于肿瘤诊断和 NIS 报告基因成像的广阔前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e40/8923191/311353e7fd4b/MOI2022-2679260.008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e40/8923191/311353e7fd4b/MOI2022-2679260.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e40/8923191/852c6071649e/MOI2022-2679260.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e40/8923191/6c0d8d569c75/MOI2022-2679260.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e40/8923191/b8bd5311d661/MOI2022-2679260.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e40/8923191/b32a114eb1cb/MOI2022-2679260.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e40/8923191/8d195843f838/MOI2022-2679260.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e40/8923191/c01a2aeb8400/MOI2022-2679260.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e40/8923191/9eb8660ff63f/MOI2022-2679260.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e40/8923191/311353e7fd4b/MOI2022-2679260.008.jpg

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