一种从人多能干细胞中区分伤害感受器、机械感受器和本体感受器的方案。
A protocol to differentiate nociceptors, mechanoreceptors, and proprioceptors from human pluripotent stem cells.
机构信息
Center for Molecular Medicine, University of Georgia, Athens, GA 30602, USA.
Department of Biochemistry and Molecular Biology, Franklin College of Arts and Sciences, University of Georgia, Athens, GA 30602, USA.
出版信息
STAR Protoc. 2022 Mar 18;3(2):101187. doi: 10.1016/j.xpro.2022.101187. eCollection 2022 Jun 17.
Human pluripotent stem cells (hPSCs) show promise for studying diseases affecting cell populations that are not easily available, including sensory neurons (SNs). Here, we present a differentiation protocol in chemically defined conditions to generate peripheral SNs from hPSCs. We describe four main steps: expansion of hPSCs, neural crest cell (NCC) differentiation, NCC dissociation and replating, and sensory neuron (SN) differentiation. This protocol enables generation of a mechanoreceptor-enriched culture or a population containing all three SN subtypes (nociceptors, mechanoreceptors, and proprioceptors). For complete details on the use and execution of this protocol, please refer to Saito-Diaz et al. (2021).
人多能干细胞（hPSCs）在研究不易获得的细胞群体相关疾病方面具有很大的应用潜力，其中包括感觉神经元（SNs）。在此，我们提出了一个在化学定义条件下分化产生人多能干细胞来源的周围感觉神经元的方案。该方案包括四个主要步骤：hPSCs 的扩增、神经嵴细胞（NCC）的分化、NCC 的分离和再铺板、以及感觉神经元（SN）的分化。该方案可产生富含机械感受器的培养物，或包含所有三种 SN 亚型（伤害感受器、机械感受器和本体感受器）的细胞群体。有关该方案的使用和执行的详细信息，请参考 Saito-Diaz 等人（2021 年）的文献。
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