Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan, Taiwan; Faculty of Applied Sciences, UCSI University, Kuala Lumpur, Malaysia.
Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan, Taiwan; Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Taoyuan, Taiwan.
J Biotechnol. 2022 May 20;350:1-10. doi: 10.1016/j.jbiotec.2022.03.008. Epub 2022 Mar 21.
Microalgae is a sustainable protein source that has been widely applied in animal feeds, functional foods, pharmaceutical, and cosmeceutical industries. Waste products could be a potential cost-saving and nutrient-rich substrate in the cultivation of microalgae for protein production. This study aims to investigate the cultivation condition of Haematococcus pluvialis for protein synthesis using synthetic brewery wastewater (BW). H. pluvialis was cultivated in the Bold's Basal Medium (BBM) mixed with synthetic BW at different concentrations. Various cultivation conditions including brewer's spent grain hydrolysate (BSGH) concentrations, pH, and light sources were studied. The molecular weight, amino acids profile and antioxidant activity of synthesized protein were determined. Fed-batch cultivation using different percentages of fresh medium replacement for enhancing protein production was investigated. The 20% fed-batch cultivation reached 27 × 10 ± 0.42 cells/mL, and 4-fold of the protein content of 64.93 ± 5.30% of dry weight was recorded on day-13. Seven essential amino acids (lysine, threonine, histidine, phenylalanine, isoleucine, leucine, methionine) were produced in fed-batch cultivation. Red LED obtained the highest DPPH radical scavenging activity of 27.47 ± 0.98%. The findings suggested that BW is a promising substrate in the cultivation of H. pluvialis to commercially produce protein for numerous industrial applications.
微藻是一种可持续的蛋白质来源，已广泛应用于动物饲料、功能性食品、制药和化妆品行业。废物可以作为微藻培养生产蛋白质的潜在节省成本和富含营养的基质。本研究旨在利用合成啤酒废水 (BW) 研究雨生红球藻生产蛋白质的培养条件。将雨生红球藻在含有不同浓度合成 BW 的 Bold 基础培养基 (BBM) 中进行培养。研究了各种培养条件，包括啤酒糟水解物 (BSGH) 浓度、pH 值和光源。测定了合成蛋白质的分子量、氨基酸组成和抗氧化活性。研究了不同新鲜培养基替代率的分批补料培养以提高蛋白质产量。20%分批补料培养达到 27×10±0.42 个细胞/mL，第 13 天记录到 64.93±5.30%干重的蛋白质含量增加了 4 倍。在分批补料培养中产生了 7 种必需氨基酸（赖氨酸、苏氨酸、组氨酸、苯丙氨酸、异亮氨酸、亮氨酸、蛋氨酸）。红色 LED 获得了最高的 DPPH 自由基清除活性，为 27.47±0.98%。研究结果表明，BW 是培养雨生红球藻以商业生产用于多种工业应用的蛋白质的有前途的基质。
