Berglund A M, Ryugo D K
Brain Res. 1986 Sep 24;383(1-2):327-32. doi: 10.1016/0006-8993(86)90034-x.
Tissue from mature rat and cat cochleas was stained with basic dyes in order to distinguish between the cell bodies of type I and II spiral ganglion neurons. This same tissue was then stained with protargol, or processed immunocytochemically using a monoclonal neurofilament antibody, RT-97. Type II neurons were intensely stained, whereas type I neurons were not. We conclude that type II neurons can be clearly distinguished by their abundant neurofilament content.
为了区分I型和II型螺旋神经节神经元的细胞体，对来自成年大鼠和猫耳蜗的组织用碱性染料进行染色。然后，对同一组织用蛋白银染色，或使用单克隆神经丝抗体RT-97进行免疫细胞化学处理。II型神经元被强烈染色，而I型神经元未被染色。我们得出结论，II型神经元可因其丰富的神经丝含量而被清楚地区分出来。
