淋巴瘤患者胸腔积液中免疫复合物的分离与解离。
Isolation and dissociation of immune complexes from pleural effusions of lymphoma patients.
作者信息
Patel G V, Satam M N, Nadkarni G R, Nadkarni J J
出版信息
Cancer Immunol Immunother. 1986;23(1):51-5. doi: 10.1007/BF00205555.
Immune complexes (IC) isolated from pleural effusions of lymphomas with favorable and unfavorable prognoses were of IgG type. These IC were further dissociated by ion exchange chromatography using 8 M urea. The antibody was found to be a high molecular weight protein (1.5 X 10(5) daltons) and reacted with antihuman IgG immunologically while a second peak obtained on ion exchange chromatography may be an antigen moiety with a molecular weight of 3.2 X 10(4) daltons as it reacted immunologically with the antibody. Strong cytoplasmic fluorescence was observed with various cell suspensions of lymphomas when reacted with the antibody preparations. The antisera raised against two different antigen fractions prepared from two lymphomas--nHL and LL showed positive fluorescence with both nHL and LL suspensions. The absorption of these rabbit antibodies with individual cell extracts or with antigen preparations also entirely blocked the cytoplasmic staining. The antigen moiety (PK-II) may have a common origin in the disease process. Pleural effusions from patients with unfavorable and favorable prognoses showed identical patterns of separation of IC components.
从预后良好和不良的淋巴瘤患者胸腔积液中分离出的免疫复合物（IC）为IgG型。这些IC通过使用8M尿素的离子交换色谱进一步解离。抗体被发现是一种高分子量蛋白质（1.5×10⁵道尔顿），与抗人IgG发生免疫反应，而在离子交换色谱上获得的第二个峰可能是分子量为3.2×10⁴道尔顿的抗原部分，因为它与抗体发生免疫反应。当与抗体制剂反应时，在各种淋巴瘤细胞悬液中观察到强烈的细胞质荧光。针对从两种淋巴瘤——非霍奇金淋巴瘤（nHL）和淋巴细胞白血病（LL）制备的两种不同抗原组分产生的抗血清，在nHL和LL悬液中均显示出阳性荧光。用单个细胞提取物或抗原制剂吸收这些兔抗血清也完全阻断了细胞质染色。抗原部分（PK-II）在疾病过程中可能有共同的起源。预后良好和不良患者的胸腔积液显示出IC组分相同的分离模式。
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