Yan Cai-Xia, Wang Kun, Zhang Bo, Xiao Li-Ying, Li Shu-Zhen, Deng Ye, Li Yan
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Hubei Key Laboratory of Incidence and Intervention of Rheumatic Diseases, Hubei Minzu University, Enshi 445000, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2022 Mar;53(2):274-280. doi: 10.12182/20220360204.
To explore the differences of oral mycobiome and bacteriome between the healthy controls (H) and oral lichen planus (OLP) patients, and the co-occurrence patterns of the salivary mycobiome and bacteriome and the association with host immunity.
Saliva samples were collected from clinical OLP patients (=35) and healthy volunteers (=18). Microbiome DNA was extracted for bacterial 16S rRNA genes sequencing and fungal internal transcribed spacer 2 (ITS2) sequencing. Bioinformatics analysis was performed on the data.The levels of IL-17 and IL-23, two pro-inflammatory cytokines, in the saliva were examined, and their correlation with the bacteria was analyzed.
There was no significant difference in the overall community structure of the mycobiome and the bacteriome between OLP patients and healthy controls. The abundance of and in the saliva bacteriome was significantly increased in the OLP group (<0.05), and the relative abundance of and in the saliva mycobiome was also significantly increased (<0.05). The co-occurrence pattern of the salivary mycobiome and bacteriome showed that the aforementioned difference was not related. However, the correlation between and bacteria was altered in the H group and the OLP group, and co-occurrence was reduced in the latter group. The level of IL-17 in the saliva was significantly increased in the OLP group. IL-17 and clinical scores were significantly correlated with the abundance of .
The increased abundance of , , , and was associated with the pathogenesis of OLP, and the changes of the microbiome co-occurrence relationship and host immunity may be involved in the pathogenesis of OLP.
探讨健康对照（H）组与口腔扁平苔藓（OLP）患者口腔真菌群落和细菌群落的差异，以及唾液真菌群落和细菌群落的共生模式及其与宿主免疫的关系。
收集临床OLP患者（n = 35）和健康志愿者（n = 18）的唾液样本。提取微生物群落DNA用于细菌16S rRNA基因测序和真菌内转录间隔区2（ITS2）测序。对数据进行生物信息学分析。检测唾液中两种促炎细胞因子IL - 17和IL - 23的水平，并分析它们与细菌的相关性。
OLP患者与健康对照之间真菌群落和细菌群落的整体群落结构无显著差异。OLP组唾液细菌群落中 和 的丰度显著增加（P < 0.05），唾液真菌群落中 和 的相对丰度也显著增加（P < 0.05）。唾液真菌群落和细菌群落的共生模式表明上述差异无相关性。然而，H组和OLP组中 与细菌之间的相关性发生改变，且后者组中共生减少。OLP组唾液中IL - 17水平显著升高。IL - 17和临床评分与 的丰度显著相关。
、 、 和 丰度增加与OLP的发病机制有关，微生物群落共生关系和宿主免疫的变化可能参与了OLP的发病机制。
