Department of Biology, Maynooth University, Co. Kildare, Ireland.
Microbiology (Reading). 2022 Mar;168(3). doi: 10.1099/mic.0.001164.
The fungal pathogen is frequently cultured from the sputum of cystic fibrosis (CF) patients along with the bacterium secretes a range of secondary metabolites, and one of these, gliotoxin, has inhibitory effects on the host immune response. The effect of culture filtrate (CuF) on fungal growth and gliotoxin production was investigated. Exposure of hyphae to cells induced increased production of gliotoxin and a decrease in fungal growth. In contrast, exposure of hyphae to CuF led to increased growth and decreased gliotoxin production. Quantitative proteomic analysis was used to characterize the proteomic response of upon exposure to CuF. Changes in the profile of proteins involved in secondary metabolite biosynthesis (e.g. gliotoxin, fumagillin, pseurotin A), and changes to the abundance of proteins involved in oxidative stress (e.g. formate dehydrogenase) and detoxification (e.g. thioredoxin reductase) were observed, indicating that the bacterial secretome had a profound effect on the fungal proteome. Alterations in the abundance of proteins involved in detoxification and oxidative stress highlight the ability of to differentially regulate protein synthesis in response to environmental stresses imposed by competitors such as . Such responses may ultimately have serious detrimental effects on the host.
真菌病原体 经常从囊性纤维化 (CF) 患者的痰液中培养出来，而 细菌会分泌一系列次级代谢产物，其中一种叫做 ，对宿主免疫反应有抑制作用。研究了 培养液 (CuF) 对真菌生长和 产生的影响。暴露于 细胞的菌丝会增加 产生并减少真菌生长。相比之下，暴露于 CuF 会导致生长增加和 产生减少。定量蛋白质组学分析用于描述 暴露于 CuF 时的蛋白质组反应。观察到参与次级代谢物生物合成（如 、菌核霉素、假丝菌素 A）的蛋白质的图谱发生变化，以及参与氧化应激（如甲酸盐脱氢酶）和解毒（如硫氧还蛋白还原酶）的蛋白质丰度发生变化，表明细菌分泌物对真菌蛋白质组有深远的影响。解毒和氧化应激相关蛋白丰度的改变突出了 能够根据竞争物（如 ）施加的环境压力差异调节蛋白质合成的能力。这种反应最终可能对宿主产生严重的不利影响。
