Reece Emma, McClean Siobhán, Greally Peter, Renwick Julie
Department of Clinical Microbiology, Trinity College Dublin, Trinity Centre for Health Science, Tallaght University Hospital, Dublin 24, Ireland.
School of Biomolecular and Biomedical Sciences, University College Dublin, Ireland.
J Microbiol Methods. 2019 Sep;164:105683. doi: 10.1016/j.mimet.2019.105683. Epub 2019 Aug 3.
Aspergillus fumigatus is the most common fungus infecting/colonising people with cystic fibrosis (CF) and can negatively impact clinical status. Diagnostic laboratories rely on culture to detect A. fumigatus which is known to be less sensitive than molecular approaches. Therefore, A. fumigatus colonisation in the CF population may be underestimated. Sputum (n = 60) from 25 children with CF were collected and A. fumigatus was detected using routine culture (CM1), enhanced culture (CM2) and ITS1 qPCR. The prevalence of A. fumigatus in this young CF population was 68% by qPCR and only 16% by CM1. CM1, CM2 and qPCR detected A. fumigatus in 8%, 22% and 53% of samples, respectively. qPCR had a 94.2% and 77.4% increased odds of detecting A. fumigatus over CM1 and CM2, respectively. Molecular methods proved superior for detecting A. fumigatus in CF sputum. A. fumigatus is likely more prevalent in early CF disease than is currently reported.
烟曲霉是感染/定植于囊性纤维化（CF）患者的最常见真菌，可对临床状况产生负面影响。诊断实验室依靠培养来检测烟曲霉，而培养法的敏感性低于分子检测方法。因此，CF人群中烟曲霉的定植情况可能被低估。收集了25名CF患儿的痰液（n = 60），并采用常规培养（CM1）、强化培养（CM2）和ITS1定量聚合酶链反应（qPCR）检测烟曲霉。在这个年轻的CF人群中，通过qPCR检测到烟曲霉的患病率为68%，而通过CM1检测仅为16%。CM1、CM2和qPCR分别在8%、22%和53%的样本中检测到烟曲霉。与CM1和CM2相比，qPCR检测到烟曲霉的几率分别增加了94.2%和77.4%。分子方法在检测CF痰液中的烟曲霉方面被证明更具优势。烟曲霉在CF疾病早期的实际患病率可能比目前报道的更高。
