Curtis Aaron, Ryan Michelle, Kavanagh Kevin
Medical Mycology Unit, Department of Biology, Maynooth University, W23 F2H6 Co. Kildare, Ireland.
J Fungi (Basel). 2023 Feb 8;9(2):222. doi: 10.3390/jof9020222.
is an opportunistic fungal pathogen capable of inducing chronic and acute infection in susceptible patients. interacts with numerous bacteria that compose the microbiota of the lung, including and , both of which are common isolates from cystic fibrosis sputum. Exposure of to culture filtrate reduced fungal growth and increased gliotoxin production. Qualitative proteomic analysis of the culture filtrate identified proteins associated with metal sequestering, enzymatic degradation and redox activity, which may impact fungal growth and development. Quantitative proteomic analysis of following exposure to culture filtrate (25% /) for 24 h revealed a reduced abundance of 1,3-beta-glucanosyltransferase (-3.97 fold), methyl sterol monooxygenase erg25B (-2.9 fold) and calcium/calmodulin-dependent protein kinase (-4.2 fold) involved in fungal development, and increased abundance of glutathione S-transferase GliG (+6.17 fold), non-ribosomal peptide synthase GliP (+3.67 fold), O-methyltransferase GliM (+3.5 fold), gamma-glutamyl acyltransferase GliK (+2.89 fold) and thioredoxin reductase GliT (+2.33 fold) involved in gliotoxin production. These results reveal that exposure of to in vivo could exacerbate infection and negatively impact patient prognosis.
是一种机会性真菌病原体，能够在易感患者中引发慢性和急性感染。它与构成肺部微生物群的多种细菌相互作用，包括 和 ，这两种细菌都是囊性纤维化痰液中的常见分离株。将 暴露于 培养滤液中会降低真菌生长并增加麦角硫因的产生。对 培养滤液进行的定性蛋白质组学分析确定了与金属螯合、酶促降解和氧化还原活性相关的蛋白质，这些可能会影响真菌的生长和发育。对 暴露于 培养滤液（25% /）24小时后的定量蛋白质组学分析显示，参与真菌发育的1,3-β-葡聚糖基转移酶（-3.97倍）、甲基甾醇单加氧酶erg25B（-2.9倍）和钙/钙调蛋白依赖性蛋白激酶（-4.2倍）丰度降低，而参与麦角硫因产生的谷胱甘肽S-转移酶GliG（+6.17倍）、非核糖体肽合成酶GliP（+3.67倍）、O-甲基转移酶GliM（+3.5倍）、γ-谷氨酰酰基转移酶GliK（+2.89倍）和硫氧还蛋白还原酶GliT（+2.33倍）丰度增加。这些结果表明，在体内将 暴露于 可能会加重感染并对患者预后产生负面影响。
